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Mitochondrial Membranes

Mitochondrial Membranes

A single mitochondrion is a bean-shaped organelle enclosed by a double-membrane system. The outer membrane of mitochondria is smooth and contains many porins - the integral membrane transporters. Porins enable free diffusion of ions and small uncharged molecules through the outer mitochondrial membrane but limit the transport of molecules larger than 5000 Daltons. Further, the outer mitochondrial membrane forms a unique structure called membrane contact sites with other subcellular organelles,...

Mitochondrial Membranes

Mitochondrial Membranes

A single mitochondrion is a bean-shaped organelle enclosed by a double-membrane system. The outer membrane of mitochondria is smooth and contains many porins - the integral membrane transporters. Porins enable free diffusion of ions and small uncharged molecules through the outer mitochondrial membrane but limit the transport of molecules larger than 5000 Daltons. Further, the outer mitochondrial membrane forms a unique structure called membrane contact sites with other subcellular organelles,...

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The inner mitochondrial membrane is the primary site of ATP synthesis. The inner membrane domain that forms a smooth layer adjacent to the outer membrane is called the inner boundary membrane. This domain contains membrane transporters that drive metabolites in and out of the mitochondria. In contrast, the inner membrane network that invaginates into the matrix space is called the cristae membrane. This domain accounts for principle mitochondrial function as it accommodates the protein...

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Related Experiment Video

Updated: May 31, 2026

Isolation of Cellular Lipid Droplets: Two Purification Techniques Starting from Yeast Cells and Human Placentas

Isolation of Cellular Lipid Droplets: Two Purification Techniques Starting from Yeast Cells and Human Placentas

Published on: April 1, 2014

Interactomic study on interaction between lipid droplets and mitochondria.

Jing Pu¹, Cheol Woong Ha, Shuyan Zhang

¹National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing, China.

|July 13, 2011

Summary

This summary is machine-generated.

Lipid droplets physically contact mitochondria and peroxisomes, revealing key protein interactions involved in yeast lipid metabolism and cellular energy homeostasis.

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Area of Science:

Cell Biology
Biochemistry
Yeast Genetics

Background:

Lipid droplets are neutral lipid storage organelles.
Their interaction with mitochondria is implicated in lipid metabolism and energy homeostasis.
The precise cellular functions and molecular mechanisms of this interaction are not fully understood.

Purpose of the Study:

To investigate the physical interactions between lipid droplets and other organelles.
To identify the molecular players involved in these interactions.
To elucidate the role of lipid droplets in cellular lipid metabolism.

Main Methods:

Transmission electron microscopy (TEM) for ultrastructural analysis.
Fluorescence imaging and reconstitution assays for in vivo and in vitro studies.
Bimolecular fluorescence complementation (BiFC) in Saccharomyces cerevisiae to map protein-protein interactions.

Main Results:

Demonstrated physical contact between lipid droplets and mitochondria in vivo and in vitro.
Generated an interactomic map of lipid droplet, mitochondria, and peroxisome contacts in yeast.
Identified lipid droplet proteins Erg6 and Pet10 as key players in 75% of detected interactions.
Discovered interactions among three pairs of lipid metabolic enzymes.

Conclusions:

Lipid droplets form physical contacts with mitochondria and peroxisomes.
Specific molecular interactions highlight the active role of lipid droplets in yeast lipid metabolism.
These findings provide insights into the functional significance of organelle interactions in cellular processes.