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Updated: May 31, 2026

Chemical Triphosphorylation of Oligonucleotides

Chemical Triphosphorylation of Oligonucleotides

Published on: June 2, 2022

Therapeutic oligonucleotides.

John Goodchild¹

¹Department of Chemistry, Worcester State University, Worcester, MA 01602-2597, USA. john.goodchild@worcester.edu

Methods in Molecular Biology (Clifton, N.J.)

|July 13, 2011

Summary

Antisense oligonucleotides, initially complex, now offer new therapeutic strategies by controlling cellular functions through binding RNA, DNA, or proteins. These nucleic acid-based approaches show promise for treating diseases.

Area of Science:

Molecular Biology
Biochemistry
Genetics

Background:

Early gene silencing efforts in the 1980s utilized antisense oligonucleotides.
Oligonucleotide pharmacology presented significant challenges, hindering therapeutic development.
Advances in understanding biochemistry and molecular biology paved the way for new applications.

Purpose of the Study:

To review the historical development of gene silencing using oligonucleotides.
To outline the biochemistry of small nucleic acids.
To explore diverse oligonucleotide-based strategies for controlling cellular functions and treating diseases.

Main Methods:

Review of historical literature on antisense oligonucleotides.
Biochemical analysis of small nucleic acids.

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Published on: July 28, 2017

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Categorization of oligonucleotide approaches based on molecular targets (RNA, DNA, proteins).

Main Results:

Early antisense oligonucleotide therapies faced pharmacological complexities.
New therapeutic avenues have emerged due to a deeper understanding of oligonucleotide biochemistry.
Oligonucleotides can target RNA (antisense, siRNA), DNA (antigene), or proteins (aptamers, decoys, CpG).

Conclusions:

Oligonucleotide-based therapies have evolved significantly from early attempts.
Diverse oligonucleotide strategies offer potential for novel disease treatments.
Continued research into oligonucleotide pharmacology is crucial for therapeutic advancement.