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Related Experiment Video

Updated: May 31, 2026

Rapid Antibody Glycoengineering in Chinese Hamster Ovary Cells

Rapid Antibody Glycoengineering in Chinese Hamster Ovary Cells

Published on: June 2, 2022

Antibody targeted siRNA delivery.

Masoud M Toloue¹, Lance P Ford

¹Bioo Scientific Corporation, 78744, Austin, TX, USA. mtoloue@biooscientific.com

Methods in Molecular Biology (Clifton, N.J.)

|July 13, 2011

Summary

Antibody-based delivery agents enable targeted delivery of small interfering RNA (siRNA) for gene silencing therapies. This method overcomes a key hurdle for in vivo RNA interference (RNAi) applications.

Area of Science:

Molecular Biology
Biotechnology
Drug Delivery

Background:

RNA interference (RNAi) is a powerful gene silencing technique.
Effective in vivo delivery of small interfering RNA (siRNA) and microRNA (miRNA) to target cells remains a significant challenge for therapeutic applications.
Commercial success of RNAi therapies depends on innovative delivery systems.

Purpose of the Study:

To develop antibody-based delivery agents for targeted in vivo delivery of siRNA.
To overcome limitations in current RNAi delivery methods for therapeutic applications.

Main Methods:

Antibodies targeting specific cell surface receptors were used.
Modified siRNAs were conjugated to antibody complexes via RNA carrier proteins.
Receptor-mediated endocytosis was utilized for cellular uptake in cultured cells and animal models.

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Last Updated: May 31, 2026

Rapid Antibody Glycoengineering in Chinese Hamster Ovary Cells

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Published on: June 2, 2022

Analyzing Tumor and Tissue Distribution of Target Antigen Specific Therapeutic Antibody

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Delivery of Therapeutic siRNA to the CNS Using Cationic and Anionic Liposomes

Delivery of Therapeutic siRNA to the CNS Using Cationic and Anionic Liposomes

Published on: July 23, 2016

Labile linking agents facilitated siRNA release post-internalization.

Main Results:

Antibody-based agents successfully delivered siRNA to specific cell types in vitro and in vivo.
The method allows conjugation of any available antibody to siRNA for targeted delivery.
Receptor-mediated endocytosis facilitated efficient cellular uptake.

Conclusions:

Antibody-mediated delivery represents a versatile strategy for targeted siRNA delivery.
This approach addresses a critical challenge in developing RNAi-based human therapeutics.
The developed method offers a flexible platform for utilizing existing antibodies in RNAi delivery systems.