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Updated: May 31, 2026

A Flow Cytometry-Based Cell Surface Protein Binding Assay for Assessing Selectivity and Specificity of an Anticancer Aptamer
Published on: September 13, 2022
Aptamer-drug conjugation for targeted tumor cell therapy
Michael J Donovan1, Ling Meng, Tao Chen
1Department of Chemistry, Shands Cancer Center, Moffitt Cancer Center, Center for Research at Bio/Nano Interface, UF Genetics Institute and McKnight Brain Institute, University of Florida, Gainesville, FL, USA. mjdonovan@ufl.edu
Abstract:
Aptamers developed for applications in cancer therapy can improve the efficacy of drug treatment and enhance molecular imaging. Aptamers for these purposes are generated from SELEX (Systematic Evolution of Ligands by EXponential enrichment), more precisely cell-based SELEX, a process described in detail in this chapter. Experimental applications are also provided for aptamer-based drugs.
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