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RNA Interference01:23

RNA Interference

RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
RNA Interference01:23

RNA Interference

RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
Experimental RNAi02:15

Experimental RNAi

RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
siRNA - Small Interfering RNAs02:30

siRNA - Small Interfering RNAs

Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the ATP-dependent...

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Related Experiment Video

Updated: May 31, 2026

Cercarial Transformation and in vitro Cultivation of Schistosoma mansoni Schistosomules
05:30

Cercarial Transformation and in vitro Cultivation of Schistosoma mansoni Schistosomules

Published on: August 16, 2011

Using RNA interference in Schistosoma mansoni.

Rita Bhardwaj1, Greice Krautz-Peterson, Patrick J Skelly

  • 1Division of Infectious Diseases, Department of Biomedical Sciences, Cummings School of Veterinary Medicine, Tufts University, North Grafton, MA, USA. Rita.Bhardwaj@Tufts.edu

Methods in Molecular Biology (Clifton, N.J.)
|July 13, 2011
PubMed
Summary

Researchers developed RNA interference (RNAi) methods to suppress gene expression in Schistosoma mansoni parasites. This molecular tool aids in understanding the parasite

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Related Experiment Videos

Last Updated: May 31, 2026

Cercarial Transformation and in vitro Cultivation of Schistosoma mansoni Schistosomules
05:30

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Published on: August 16, 2011

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Published on: March 1, 2022

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Area of Science:

  • Parasitology
  • Molecular Biology
  • Genetics

Background:

  • Schistosomes are parasitic worms causing significant global health issues.
  • Understanding schistosome molecular biology is crucial for developing control strategies.
  • RNA interference (RNAi) is a powerful gene silencing technique.

Purpose of the Study:

  • To describe methods for applying RNAi in Schistosoma mansoni.
  • To enable gene-specific expression suppression in parasite intra-mammalian stages.

Main Methods:

  • Isolation and culturing of Schistosoma mansoni.
  • Preparation and delivery of gene-specific double-stranded RNA (dsRNA).
  • Monitoring gene expression outcomes post-dsRNA treatment.

Main Results:

  • Established protocols for RNAi application in Schistosoma mansoni.
  • Demonstrated successful gene expression suppression using RNAi.

Conclusions:

  • RNAi technology is a promising tool for analyzing gene function in Schistosoma mansoni.
  • This methodology can accelerate research into this globally important parasite.