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Related Experiment Video

Updated: May 31, 2026

Coin Cell Battery Chamber Design for Low-temperature Operando Experiments

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Published on: February 17, 2026

Boyden chamber.

Marco Falasca¹, Claudio Raimondi, Tania Maffucci

¹Barts and The London School of Medicine and Dentistry, Blizard Institute of Cell and Molecular Science, London, UK. m.falasca@qmul.ac.uk

Methods in Molecular Biology (Clifton, N.J.)

|July 13, 2011

Summary

This summary is machine-generated.

The Boyden chamber assay measures cell migration and invasion. This method is crucial for studying endothelial and cancer cell movement through barriers, aiding cancer research.

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Area of Science:

Cell Biology
Biotechnology
Cancer Research

Background:

The Boyden chamber assay is a widely adopted method for evaluating cell motility and invasion.
Originally developed for leukocyte chemotaxis, it assesses the ability of cells to traverse a physical barrier.
Variations exist, including adaptations for studying tumor cell invasion using extracellular matrix proteins.

Purpose of the Study:

To detail a method for measuring human endothelial and cancer cell migration and invasion.
To provide a protocol applicable to various Boyden chamber configurations.
To highlight the utility of the Boyden chamber in cancer and cell biology research.

Main Methods:

Utilizes a Boyden chamber with two compartments separated by a porous membrane.
Cells migrate or invade across the membrane, which can be coated with extracellular matrix components.
The method is specifically tailored for assessing human endothelial and cancer cell behavior.

Main Results:

Quantifies the migratory and invasive capacity of endothelial and cancer cells.
Demonstrates the adaptability of the Boyden chamber for different cell types and experimental conditions.
Provides a standardized approach for comparative studies of cell motility.

Conclusions:

The Boyden chamber assay is a versatile and effective tool for studying cell migration and invasion.
This method is particularly valuable for investigating the invasive potential of cancer cells.
The described protocol facilitates research in cell biology, oncology, and drug development.