Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Role of Matrix Metalloproteases in Degradation of ECM01:23

Role of Matrix Metalloproteases in Degradation of ECM

Matrix metalloproteases (MMPs) are enzymes involved in the hydrolysis of proteins and glycoproteins of the extracellular matrix. MMPs are essential for the migration and proliferation of cells through the dense matrix network, throughout embryonic development, and throughout morphogenesis. The first MMP activity discovered was a collagenase in a tadpole's tail undergoing metamorphosis. The active collagen deposition and modifications lead to the morphogenesis of tadpoles into the adult body.
A...
Cancer Cell Migration through Invadopodia01:35

Cancer Cell Migration through Invadopodia

Invadosome is a broad category of cell surface structures with proteolytic activity that  degrades the extracellular matrix (ECM). Invadosomes are present in normal cell types, including macrophages, endothelial cells, and neurons, as well as tumor cells. Although the macrophage podosomes and tumor cell invadopodia are classified as invadosomes, they have different structures, molecular pathways, and functions. Podosomes are short structures that last for a few minutes. However, invadopodia can...
Microtubule Instability02:17

Microtubule Instability

Microtubules are hollow cylindrical filaments having a diameter of approximately 25 nm and a length that varies from 200 nm to 25 μm. GTP-bound tubulin subunits form αβ-heterodimers for microtubule assembly. These core building blocks interact longitudinally, polymerizing into protofilaments. The protofilaments then interact with one another through lateral bonding forces to form stable cylindrical microtubules. These cylindrical filaments are dynamic as they undergo repeated assembly and...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Multi-omic analysis reveals nitric oxide dependent remodeling in classically activated macrophages and identifies negative regulation mediated by AKR1A1.

Redox biology·2026
Same author

Evidence that xylazine disrupts skin homeostasis by acting on epithelial cells through the kappa opioid receptor.

Disease models & mechanisms·2026
Same author

The paracrine factor myeloid derived growth factor regulates the inflammatory fate and motility of human induced pluripotent stem cell-derived neutrophils.

Journal of immunology (Baltimore, Md. : 1950)·2026
Same author

Leukocyte-epithelial physical contacts mediate interstitial migration in vivo.

Research square·2026
Same author

Multi-omic analysis reveals nitric oxide dependent remodeling in classically activated macrophages and identifies negative regulation mediated by AKR1A1.

bioRxiv : the preprint server for biology·2026
Same author

Population-Specific Transcriptomic Shifts Underlie Secondary Metabolic Diversification in <i>Aspergillus flavus</i> and the Domestication of <i>Aspergillus oryzae</i>.

bioRxiv : the preprint server for biology·2025

Related Experiment Video

Updated: May 31, 2026

Quantitative Measurement of Invadopodia-mediated Extracellular Matrix Proteolysis in Single and Multicellular Contexts
14:23

Quantitative Measurement of Invadopodia-mediated Extracellular Matrix Proteolysis in Single and Multicellular Contexts

Published on: August 27, 2012

Imaging podosome dynamics and matrix degradation.

Taylor W Starnes1, Christa L Cortesio, Anna Huttenlocher

  • 1Department of Medical Microbiology and Immunology, University of Wisconsin-Madison, Madison, WI, USA.

Methods in Molecular Biology (Clifton, N.J.)
|July 13, 2011
PubMed
Summary
This summary is machine-generated.

This study details methods for visualizing and measuring podosomes, crucial for macrophage invasion. These techniques enable the quantification of podosome dynamics and matrix degradation, advancing research on cell migration.

More Related Videos

Imaging of Podocytic Proteins Nephrin, Actin, and Podocin with Expansion Microscopy
06:18

Imaging of Podocytic Proteins Nephrin, Actin, and Podocin with Expansion Microscopy

Published on: April 23, 2021

2D and 3D Matrices to Study Linear Invadosome Formation and Activity
12:25

2D and 3D Matrices to Study Linear Invadosome Formation and Activity

Published on: June 2, 2017

Related Experiment Videos

Last Updated: May 31, 2026

Quantitative Measurement of Invadopodia-mediated Extracellular Matrix Proteolysis in Single and Multicellular Contexts
14:23

Quantitative Measurement of Invadopodia-mediated Extracellular Matrix Proteolysis in Single and Multicellular Contexts

Published on: August 27, 2012

Imaging of Podocytic Proteins Nephrin, Actin, and Podocin with Expansion Microscopy
06:18

Imaging of Podocytic Proteins Nephrin, Actin, and Podocin with Expansion Microscopy

Published on: April 23, 2021

2D and 3D Matrices to Study Linear Invadosome Formation and Activity
12:25

2D and 3D Matrices to Study Linear Invadosome Formation and Activity

Published on: June 2, 2017

Area of Science:

  • Cell Biology
  • Immunology
  • Biophysics

Background:

  • Invasive cell migration is essential for leukocyte trafficking into tissues.
  • Podosomes are key matrix-degrading adhesive structures enabling macrophage migration and invasion.

Purpose of the Study:

  • To describe methods for imaging and quantifying podosomes in primary human macrophages and THP-1 cells.
  • To outline detailed methods for live imaging of dynamic podosomes and quantifying their turnover rates.
  • To discuss quantitative analysis of matrix degradation using fluorescent-gelatin-coated coverslips.

Main Methods:

  • Imaging and quantification of podosomes in primary human macrophages and differentiated THP-1 cells.
  • Live imaging techniques for observing dynamic podosome behavior.
  • Quantification of podosome turnover rates and matrix degradation on fluorescent-gelatin substrates.

Main Results:

  • Established protocols for visualizing and quantifying podosomes in macrophages.
  • Demonstrated methods for assessing podosome dynamics and turnover in real-time.
  • Provided a framework for quantitative analysis of matrix degradation by podosomes.

Conclusions:

  • The described methods facilitate detailed study of podosome function in macrophage migration.
  • Accurate quantification of podosome dynamics and matrix degradation is achievable.
  • These techniques will aid further research into the mechanisms of invasive cell migration.