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Related Experiment Video

Updated: May 31, 2026

Analyzing In Vivo Cell Migration using Cell Transplantations and Time-lapse Imaging in Zebrafish Embryos
11:39

Analyzing In Vivo Cell Migration using Cell Transplantations and Time-lapse Imaging in Zebrafish Embryos

Published on: April 29, 2016

Measuring inflammatory cell migration in the zebrafish.

Philip M Elks1, Catherine A Loynes, Stephen A Renshaw

  • 1MRC Centre for Developmental and Biomedical Genetics, The University of Sheffield, Sheffield, UK.

Methods in Molecular Biology (Clifton, N.J.)
|July 13, 2011
PubMed
Summary

Inflammatory cell migration is crucial for healing and fighting infection. Zebrafish models allow researchers to visualize neutrophil movement in vivo, advancing our understanding of inflammation.

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Area of Science:

  • Immunology
  • Developmental Biology
  • Cell Biology

Background:

  • Inflammatory cell migration is vital for host defense and tissue repair.
  • Understanding inflammation resolution is critical for maintaining health.
  • Studying complex cell-cell interactions in vitro is challenging.

Purpose of the Study:

  • To investigate the in vivo dynamics of inflammatory cell migration.
  • To utilize a whole-organism model for studying inflammation.
  • To elucidate the cellular processes underlying inflammation and its resolution.

Main Methods:

  • Utilizing near-transparent zebrafish (Danio rerio) larvae as an in vivo model.
  • Employing a transgenic zebrafish line Tg(mpx:GFP)i114 to fluorescently label neutrophils.

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Related Experiment Videos

Last Updated: May 31, 2026

Analyzing In Vivo Cell Migration using Cell Transplantations and Time-lapse Imaging in Zebrafish Embryos
11:39

Analyzing In Vivo Cell Migration using Cell Transplantations and Time-lapse Imaging in Zebrafish Embryos

Published on: April 29, 2016

High-resolution Cell Transplantation in Embryonic and Larval Zebrafish
06:08

High-resolution Cell Transplantation in Embryonic and Larval Zebrafish

Published on: July 5, 2024

Invasive Behavior of Human Breast Cancer Cells in Embryonic Zebrafish
09:22

Invasive Behavior of Human Breast Cancer Cells in Embryonic Zebrafish

Published on: April 25, 2017

  • Observing neutrophil behavior at a single-cell level using advanced imaging techniques.

    • Main Results:

    • Demonstrated the feasibility of studying inflammatory cell migration in vivo using zebrafish.
    • Enabled visualization of neutrophil dynamics throughout inflammatory processes.
    • Provided a platform for detailed analysis of cellular behaviors during inflammation.

    Conclusions:

    • Zebrafish larvae offer a powerful model for studying complex inflammatory processes.
    • In vivo imaging of neutrophils provides novel insights into inflammation dynamics.
    • This approach enhances our understanding of cellular mechanisms in immunity and healing.