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Antibody-free detection of protein tyrosine nitration in tissue sections

Rosalina Wisastra1, Klaas Poelstra, Rainer Bischoff

  • 1Pharmaceutical Gene Modulation, Groningen Research Institute of Pharmacy, University of Groningen, P. O. Box 196, 9700 AD Groningen, The Netherlands.

Chembiochem : a European Journal of Chemical Biology
|July 13, 2011
PubMed
Abstract

No abstract available in PubMed .

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Western Blotting

Western blotting is an analytical technique for protein identification. It has various applications in immunology and medicine, including detecting diseases like bovine spongiform encephalopathy, mad cow disease, and human and feline immunodeficiency virus from biological samples.
The technique begins with separating proteins from the sample using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), followed by protein transfer, immunoblotting, and finally, protein detection.