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Related Concept Videos

RNA Interference01:23

RNA Interference

RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
RNA Interference01:23

RNA Interference

RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
Experimental RNAi02:15

Experimental RNAi

RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
siRNA - Small Interfering RNAs02:30

siRNA - Small Interfering RNAs

Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the ATP-dependent...
Small interfering RNAs (siRNA)02:30

Small interfering RNAs (siRNA)

Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the ATP-dependent...
piRNA - Piwi-interacting RNAs02:57

piRNA - Piwi-interacting RNAs

PIWI-interacting RNAs, or piRNAs, are the most abundant short non-coding RNAs. More than 20,000 genes have been found in humans that code for piRNAs while only 2000 genes have been found for miRNAs. piRNAs can act at the transcriptional and post-transcriptional levels and have a vital role in silencing transposable elements present in germ cells. They are also involved in epigenetic silencing and activation. Previously, they were thought to function only in germ cells but new evidence suggests...

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Related Experiment Video

Updated: May 31, 2026

Porous Silicon Microparticles for Delivery of siRNA Therapeutics
08:31

Porous Silicon Microparticles for Delivery of siRNA Therapeutics

Published on: January 15, 2015

Polymers in small-interfering RNA delivery.

Kaushik Singha1, Ran Namgung, Won Jong Kim

  • 1Department of Chemistry, BK School of Molecular Science, Polymer Research Institute, Pohang University of Science and Technology, Pohang, Korea.

Nucleic Acid Therapeutics
|July 14, 2011
PubMed
Summary
This summary is machine-generated.

This review explores nonviral polymer vectors for efficient small interfering RNA (siRNA) delivery. Strategies focus on modifying polymers and nanoparticles for targeted, stimuli-responsive therapeutic outcomes.

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Assembly and Characterization of Polyelectrolyte Complex Micelles
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Assembly and Characterization of Polyelectrolyte Complex Micelles

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Last Updated: May 31, 2026

Porous Silicon Microparticles for Delivery of siRNA Therapeutics
08:31

Porous Silicon Microparticles for Delivery of siRNA Therapeutics

Published on: January 15, 2015

Preparation of Neutrally-charged, pH-responsive Polymeric Nanoparticles for Cytosolic siRNA Delivery
09:09

Preparation of Neutrally-charged, pH-responsive Polymeric Nanoparticles for Cytosolic siRNA Delivery

Published on: May 2, 2019

Assembly and Characterization of Polyelectrolyte Complex Micelles
08:44

Assembly and Characterization of Polyelectrolyte Complex Micelles

Published on: March 2, 2020

Area of Science:

  • Biotechnology
  • Nanomedicine
  • Molecular Biology

Background:

  • Small interfering RNA (siRNA) shows therapeutic potential but requires effective delivery systems.
  • Nonviral vectors offer a safer alternative to viral methods for gene delivery.
  • Polymeric materials are extensively investigated for siRNA encapsulation and targeted delivery.

Purpose of the Study:

  • To review current strategies for efficient siRNA delivery using nonviral polymeric vectors.
  • To discuss the principles, modifications, and applications of various polymeric vectors in siRNA therapeutics.
  • To highlight advancements in stimuli-responsive and targeted delivery systems for enhanced therapeutic efficacy.

Main Methods:

  • Review of literature on nonviral vector-mediated siRNA delivery.
  • Analysis of polymeric materials including polyethylenimine, PLGA, polypeptides, chitosan, cyclodextrins, and dendrimers.
  • Discussion of modifications for stimuli-responsiveness and targeted delivery using nanoconstructs.

Main Results:

  • Various polymers (PEI, PLGA, etc.) demonstrate potential for siRNA delivery.
  • Structural and functional modifications enhance vector performance.
  • Incorporation of nanoparticles (CNTs, AuNPs, silica NPs) improves targeting and responsiveness.

Conclusions:

  • Nonviral polymeric vectors are promising for efficient and targeted siRNA delivery.
  • Strategic modifications are key to overcoming delivery challenges and achieving therapeutic outcomes.
  • Continued research in nanoconstructs integration will advance siRNA-based therapies.