Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Next-generation Sequencing

Next-generation Sequencing

The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features.

Evolutionary Relationships through Genome Comparisons

Evolutionary Relationships through Genome Comparisons

Genome comparison is one of the excellent ways to interpret the evolutionary relationships between organisms. The basic principle of genome comparison is that if two species share a common feature, it is likely encoded by the DNA sequence conserved between both species. The advent of genome sequencing technologies in the late 20th century enabled scientists to understand the concept of conservation of domains between species and helped them to deduce evolutionary relationships across diverse...

Sanger Sequencing

Sanger Sequencing

DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...

Gene Evolution - Fast or Slow?

Gene Evolution - Fast or Slow?

The genomes of eukaryotes are punctuated by long stretches of sequence which do not code for proteins or RNAs. Although some of these regions do contain crucial regulatory sequences, the vast majority of this DNA serves no known function. Typically, these regions of the genome are the ones in which the fastest change, in evolutionary terms, is observed, because there is typically little to no selection pressure acting on these regions to preserve their sequences.
In contrast, regions which code...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Optimizing third-line and beyond therapies for metastatic colorectal cancer in Taiwan: an evidence-based consensus.

Therapeutic advances in medical oncology·2026

Same author

Achieving Targeted Combined Endpoints in Patients With Atopic Dermatitis: A Real-World Study.

The Journal of dermatology·2026

Same author

Cunninghamia lanceolata genome illuminates the evolutionary dynamics of gymnosperms.

Cell reports·2026

Same author

Correction to "Prominent Vascularization Capacity of Mesenchymal Stem Cells in Collagen-Gold Nanocomposites".

ACS applied materials & interfaces·2026

Same author

Corrigendum to "Functional engineered mesenchymal stem cells with fibronectin‑gold composite coated catheters for vascular tissue regeneration" [Nanomed: Nanotechnol Biol Med 14 (2018) 699-711].

Nanomedicine : nanotechnology, biology, and medicine·2026

Same author

GLR channels are required for rhythmic scent emission in Phalaenopsis bellina.

The Plant journal : for cell and molecular biology·2026

Search research articles

Related Experiment Video

Updated: May 31, 2026

Pyrosequencing: A Simple Method for Accurate Genotyping

Pyrosequencing: A Simple Method for Accurate Genotyping

Published on: January 8, 2008

Gene discovery using next-generation pyrosequencing to develop ESTs for Phalaenopsis orchids.

Yu-Yun Hsiao¹, Yun-Wen Chen, Shi-Ching Huang

¹Department of Life Sciences, National Cheng Kung University, Tainan 701, Taiwan.

|July 14, 2011

Summary

This study used 454 pyrosequencing to characterize the Phalaenopsis orchid transcriptome, generating a valuable expressed sequence tag (EST) dataset for gene discovery in orchids.

More Related Videos

Fluorescence-microscopy Screening and Next-generation Sequencing: Useful Tools for the Identification of Genes Involved in Organelle Integrity

Fluorescence-microscopy Screening and Next-generation Sequencing: Useful Tools for the Identification of Genes Involved in Organelle Integrity

Published on: April 13, 2012

Optimization and Comparative Analysis of Plant Organellar DNA Enrichment Methods Suitable for Next-generation Sequencing

Optimization and Comparative Analysis of Plant Organellar DNA Enrichment Methods Suitable for Next-generation Sequencing

Published on: July 28, 2017

Related Experiment Videos

Last Updated: May 31, 2026

Pyrosequencing: A Simple Method for Accurate Genotyping

Pyrosequencing: A Simple Method for Accurate Genotyping

Published on: January 8, 2008

Fluorescence-microscopy Screening and Next-generation Sequencing: Useful Tools for the Identification of Genes Involved in Organelle Integrity

Fluorescence-microscopy Screening and Next-generation Sequencing: Useful Tools for the Identification of Genes Involved in Organelle Integrity

Published on: April 13, 2012

Optimization and Comparative Analysis of Plant Organellar DNA Enrichment Methods Suitable for Next-generation Sequencing

Optimization and Comparative Analysis of Plant Organellar DNA Enrichment Methods Suitable for Next-generation Sequencing

Published on: July 28, 2017

Area of Science:

Plant genomics
Orchid biology
Transcriptome sequencing

Background:

Orchids are highly diverse angiosperms with limited genomic resources.
Phalaenopsis orchids are ecologically significant and economically important in floriculture.
Genomic characterization is crucial for understanding orchid biology.

Purpose of the Study:

To perform a global characterization of the Phalaenopsis transcriptome.
To generate a comprehensive expressed sequence tag (EST) dataset.
To facilitate gene discovery in orchids.

Main Methods:

Massively parallel 454 pyrosequencing was employed.
RNA was pooled from diverse Phalaenopsis tissues, developmental stages, and stress conditions.
Expressed sequence tags (ESTs) were assembled into unigenes and annotated.

Main Results:

206,960 expressed sequence tags (ESTs) were generated.
8,233 contigs and 34,630 singletons were assembled.
22,234 unique genes were identified, with over 780 putative transcription factors.

Conclusions:

454 pyrosequencing effectively identified a large set of unigenes in Phalaenopsis.
The developed EST dataset is a vital resource for Phalaenopsis and other orchid species.
This resource bridges the gap in genomic information for non-model orchid species.