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Published on: September 17, 2021
Chlorogenic acid protects MSCs against oxidative stress by altering FOXO family genes and activating intrinsic
Shiyong Li1, Hetao Bian, Zhe Liu
1Key Laboratory of Molecular Medicine, Second Affiliated Hospital of Nanchang University, Nanchang City, China.
Abstract:
Chlorogenic acid as an antioxidant exists widely in edible and medicinal plants, and can protect cell against apoptosis induced by oxidative stress. However, its molecular mechanisms remain largely unknown. Here, we showed that Chlorogenic acid suppressed reactive oxygen species increase by activation of Akt phosphorylation,and increased FOXO family genes and anti-apoptotic protein Bcl-2 expression in MSCs culturing under oxidative stress. In addition, PI-3Kinase Inhibitor (2-(4-Morpholinyl)-8-phenyl-4H-1-benzopyran-4-one, LY294002) could suppress the Chlorogenic acid-induced: (1) the cellular protective role, (2) the increase of the FOXO family genes expression, (3) increased expression of Bcl-2. These results suggested that Chlorogenic acid protected MSCs against apoptosis via PI3K/AKT signal and FOXO family genes.
Insights
Chlorogenic acid protects stem cells from oxidative stress by activating the PI3K/AKT pathway, increasing protective gene expression. This reveals key molecular mechanisms for its antioxidant effects.
Area of Science:
- Biochemistry
- Cell Biology
- Pharmacology
Background:
- Chlorogenic acid is a widely found plant antioxidant.
- It protects cells from oxidative stress-induced apoptosis.
- Its precise molecular mechanisms are not fully understood.
Purpose of the Study:
- To elucidate the molecular mechanisms by which chlorogenic acid protects mesenchymal stem cells (MSCs) against oxidative stress.
- To investigate the role of the PI3K/AKT pathway and FOXO family genes in chlorogenic acid's protective effects.
Main Methods:
- MSCs were cultured under oxidative stress conditions.
- The effects of chlorogenic acid on reactive oxygen species (ROS) levels, Akt phosphorylation, and gene expression (FOXO family, Bcl-2) were analyzed.
- The PI-3Kinase inhibitor LY294002 was used to probe the involvement of the PI3K/AKT pathway.
Main Results:
- Chlorogenic acid suppressed ROS increase via Akt phosphorylation.
- It upregulated FOXO family genes and the anti-apoptotic protein Bcl-2.
- Inhibition of PI3K/AKT pathway by LY294002 blocked these protective effects.
Conclusions:
- Chlorogenic acid protects MSCs from apoptosis through the PI3K/AKT signaling pathway.
- FOXO family genes are critical mediators in chlorogenic acid's cytoprotective action.
- This study clarifies the molecular basis of chlorogenic acid's antioxidant properties in stem cells.
