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Updated: May 31, 2026

Biosensor for Detection of Antibiotic Resistant Staphylococcus Bacteria

Biosensor for Detection of Antibiotic Resistant Staphylococcus Bacteria

Published on: May 8, 2013

Methicillin-resistant Staphylococcus aureus vancomycin susceptibility testing: methodology correlations, temporal

Sebastiaan J van Hal¹, Thelma Barbagiannakos, Mark Jones

¹Department of Microbiology & Infectious Diseases, Sydney South West Pathology Service-Liverpool, South Western Sydney Local Health Network, NSW, Sydney, Australia. sebastian.vanhal@sswahs.nsw.gov.au

The Journal of Antimicrobial Chemotherapy

|July 14, 2011

Summary

Vancomycin MIC testing methods show moderate correlation, with discrepancies observed between Etest, Vitek2, and broth microdilution. Temporal trends in MICs require cautious interpretation due to methodology dependence.

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Genotyping of Staphylococcus aureus by Ribosomal Spacer PCR (RS-PCR)

Genotyping of Staphylococcus aureus by Ribosomal Spacer PCR (RS-PCR)

Published on: November 4, 2016

Area of Science:

Clinical Microbiology
Infectious Diseases
Pharmacology

Background:

Vancomycin is a critical antibiotic for treating methicillin-resistant Staphylococcus aureus (MRSA) infections.
Accurate Minimum Inhibitory Concentration (MIC) testing is essential for guiding vancomycin therapy.
Understanding MIC creep and inter-method variability is crucial for effective treatment strategies.

Purpose of the Study:

To assess the correlation between different vancomycin MIC testing methods.
To investigate the phenomenon of MIC creep in MRSA isolates.
To evaluate the impact of testing methodology on observed MIC trends.

Main Methods:

Analysis of 417 MRSA bloodstream isolates from 1997-2008.
Susceptibility testing using Etest, Vitek2, and broth microdilution (BMD).
Phenotypic classification using Pulsed-Field Gel Electrophoresis (PFGE).

Main Results:

Moderate correlations were found between BMD, Etest, and Vitek2 (Spearman's coefficients 0.33-0.50).
Etest generally yielded MICs one dilution higher, Vitek2 one dilution lower than BMD.
MIC creep was method-dependent; BMD MICs ≥2 mg/L decreased over time, while modal MIC remained stable.

Conclusions:

Suboptimal correlation exists between vancomycin MIC testing methodologies.
Temporal MIC trends must be interpreted cautiously due to variations in testing methods and analytical approaches.
Standardization of MIC testing is needed for reliable monitoring of vancomycin resistance.