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Bacterial Artificial Chromosomes: A Functional Genomics Tool for the Study of Positive-strand RNA Viruses
Published on: December 29, 2015
Genotype v Japanese encephalitis virus is emerging.
Ming-Hua Li1, Shi-Hong Fu, Wei-Xin Chen
1State Key Laboratory for Infectious Disease Prevention and Control, Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, China.
Plos Neglected Tropical Diseases
|July 14, 2011
Summary
Japanese encephalitis virus (JEV) genotype V is emerging in endemic areas. Increased surveillance for genotype V JEV is crucial for public health in affected nations.
Area of Science:
- Virology
- Epidemiology
- Public Health
Background:
- Japanese encephalitis (JE) is a significant global health concern and the leading cause of viral encephalitis worldwide.
- Japanese encephalitis virus (JEV) is classified into five genotypes based on its envelope (E) gene nucleotide sequence.
- Genotype V JEV is currently represented solely by the Muar strain, isolated in 1952.
Purpose of the Study:
- To characterize a newly isolated JEV strain, XZ0934, from China.
- To determine the genotypic classification of the XZ0934 strain.
- To assess the implications of genotype V JEV emergence in endemic regions.
Main Methods:
- Isolation of JEV from Culex tritaeniorhynchus mosquitoes in China.
- Determination of the complete nucleotide and amino acid sequences of the XZ0934 JEV strain.
- Phylogenetic analysis using complete genome and structural gene nucleotide sequences.
Main Results:
- The XZ0934 strain exhibited significant nucleotide (20.3–21.4%) and amino acid (8.4–10.0%) divergence from JEV genotypes I–IV.
- Phylogenetic analysis confirmed that the XZ0934 strain belongs to genotype V.
- This finding indicates the presence and potential emergence of genotype V JEV.
Conclusions:
- The XZ0934 strain represents a second isolate of JEV genotype V.
- Genotype V JEV appears to be emerging in areas where JEV is endemic.
- Enhanced surveillance and diagnostics for genotype V JEV are critical for public health in endemic countries.
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