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Temperature effect on the shear-induced cell damage in biofabrication.
Ming G Li1, Xiao Y Tian, Xiongbiao Chen
1Department of Mechanical Engineering, University of Saskatchewan, Saskatoon, Canada.
Artificial Organs
|July 15, 2011
Summary
This study reveals temperature significantly impacts shear-induced cell damage during biofabrication. Understanding this relationship helps preserve cell viability in bioprocessing.
Area of Science:
- Biomaterials Engineering
- Cellular Mechanics
- Bioprocessing Technology
Background:
- Biofabrication processes often involve varying temperatures and mechanical forces.
- Shear stress is a known cause of cell damage in these processes.
- The effect of temperature on shear-induced cell damage remains under-investigated.
Purpose of the Study:
- To investigate the influence of temperature on shear-induced cell damage.
- To establish a quantitative cell damage law relating temperature and cell damage percentage.
- To model cell damage during biofabrication at different temperatures.
Main Methods:
- Experimental investigation using a cone-and-plate rheometer.
- Development of a cell damage law based on experimental data.
- Modeling of cell damage in a biofabrication process using pressurized air dispensing.
Main Results:
- A quantitative relationship between temperature and shear-induced cell damage was established.
- The developed cell damage law accurately predicted cell damage in a biofabrication model.
- Model predictions showed good agreement with experimental results.
Conclusions:
- Temperature significantly influences shear-induced cell damage in biofabrication.
- The established cell damage law provides an effective method for assessing temperature effects.
- This research offers insights for preserving cell viability in biofabrication processes.
