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Updated: May 31, 2026

Optimizing Tubulin Yield from Porcine Brain Tissue
Published on: October 11, 2024
3-(3-Phenoxybenzyl)amino-β-carboline: a novel antitumor drug targeting α-tubulin
Reiko Ikeda1, Masaki Kurosawa, Takazumi Okabayashi
1Department of Pure and Applied Chemistry, Faculty of Science and Technology, Tokyo University of Science (RIKADAI), Noda, Chiba 278-8510, Japan.
Abstract:
3-(3-Phenoxybenzyl)amino-β-carboline 2h showed extremely-high activity; the IC(50) value was 0.074 μM. To verify 2h-induced cell death types, we observed the chromatin condensation, the DNA fragmentation and activated caspase-3 using Hoechst 33342, agarose electrophoresis and western blot, and suggesting 2h-induced cell death type was apoptosis. Flow cytometry showed that 2h-treated cell was induced SubG1 cell population after G2/M cell cycle arrest. In addition, using affinity chromatography and peptide mass fingerprinting, we found that interacting protein with this compound was α-tubulin protein.
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