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Whole Genome Sequencing of Candida glabrata for Detection of Markers of Antifungal Drug Resistance
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Whole Genome Sequencing of Candida glabrata for Detection of Markers of Antifungal Drug Resistance

Published on: December 28, 2017

Rapid discrimination between Candida glabrata, Candida nivariensis, and Candida bracarensis by use of a singleplex

A Enache-Angoulvant1, J Guitard, F Grenouillet

  • 1APHP, Hôpital Bicêtre, Service de Bactériologie-Virologie-Parasitologie, Laboratoire de Parasitologie-Mycologie, Kremlin-Bicêtre 94275, France. adela.angoulvant@bct.aphp.fr

Journal of Clinical Microbiology
|July 15, 2011
PubMed

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Real Time RT-PCR02:57

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...