Experimental pestivirus infections in newborn goat kids

Two experiments were carried out in which 37 healthy newborn goat kids were inoculated with a non-cytopathic ovine (BDV) or a cytopathic bovine pestivirus (BVDV) by intramuscular or intracerebral injection. No kids showed signs of disease or gross lesions which could be attributed to these viruses, but inoculated kids had lower mean growth rates than the controls. Significant histological changes in the CNS of 14 kids were restricted largely to the white matter and consisted mainly of hypercellular foci comprising microglial/histiocytic cells and mild perivascular infiltration by mononuclear cells. Varying degrees of infiltration of the myocardium by lymphocytes and plasma cells were observed. All kids remained negative for neutralizing antibodies against pestivirus until 2 to 3 weeks after infection. Titres increased during the following weeks. Pestiviruses were recovered from kids necropsied 10 days after inoculation, but not from any kids killed 20 days after inoculation or later. Non-cytopathic virus was isolated from various tissues of four kids that had received BDV and three kids that had been given BVDV. Cytopathic viruses were not recovered from any kids. Mean white blood cell counts in all kids were within the normal range at 4 and 8 weeks after inoculation. The lymphocyte response to stimulation by phytohaemagglutinin was significantly increased on both sampling occasions in the BDV-inoculated kids, while in the BVDV-inoculated animals, a similar increase was seen only at 8 weeks.