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Mitosis and Cytokinesis

Mitosis and Cytokinesis

In eukaryotes, the cell division cycle is divided into distinct, coordinated cellular processes that include cell growth, DNA replication/chromosome duplication, chromosome distribution to daughter cells, and finally, cell division. The cell cycle is tightly regulated by its regulatory systems as well as extracellular signals that affect cell proliferation.
The processes of the cell cycle occur over approximately 24 hours (in typical human cells) and in two major distinguishable stages. The...

Mitosis and Cytokinesis

Mitosis and Cytokinesis

In eukaryotes, the cell division cycle is divided into distinct, coordinated cellular processes that include cell growth, DNA replication/chromosome duplication, chromosome distribution to daughter cells, and finally, cell division. The cell cycle is tightly regulated by its regulatory systems as well as extracellular signals that affect cell proliferation.
The processes of the cell cycle occur over approximately 24 hours (in typical human cells) and in two major distinguishable stages. The...

Meiosis vs. Mitosis

Meiosis vs. Mitosis

Cell division is necessary for growth and reproduction in organisms. Mitosis aids cell growth and development by dividing somatic cells. In contrast, meiosis causes the division of germ cells and plays an essential role in sexual reproduction. Due to their unique functional requirements, mitosis and meiosis differ from each other in multiple aspects.
Before the start of mitosis and meiosis I, the cell synthesizes DNA, resulting in two homologous copies of each chromosome. DNA synthesis is...

The Mitotic Spindle

The Mitotic Spindle

The mitotic spindle—or spindle apparatus—is a eukaryotic, cytoskeletal structure made up of long protein fibers called microtubules. Formed during cell division, the spindle separates sister chromatids and moves them to opposite ends of a parental cell, where the now individual chromosomes are distributed to two daughter cell nuclei.
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The Mitotic Spindle

The mitotic spindle—or spindle apparatus—is a eukaryotic, cytoskeletal structure made up of long protein fibers called microtubules. Formed during cell division, the spindle separates sister chromatids and moves them to opposite ends of a parental cell, where the now individual chromosomes are distributed to two daughter cell nuclei.
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Related Experiment Video

Updated: May 31, 2026

Combining Mitotic Cell Synchronization and High Resolution Confocal Microscopy to Study the Role of Multifunctional Cell Cycle Proteins During Mitosis

Combining Mitotic Cell Synchronization and High Resolution Confocal Microscopy to Study the Role of Multifunctional Cell Cycle Proteins During Mitosis

Published on: December 5, 2017

Large-scale mitotic cell synchronization.

Kalyan Dulla¹, Anna Santamaria

¹Department of Molecular Diagnostics, Philips Research, Eindhoven, The Netherlands. kalyan.dulla@philips.com

Methods in Molecular Biology (Clifton, N.J.)

|July 15, 2011

Summary

Synchronizing cell populations is crucial for studying cell cycle regulation. This work details methods for large-scale isolation of mitotic mammalian cells, optimizing enrichment at specific cell cycle stages.

Area of Science:

Cell Biology
Molecular Biology

Background:

Cell cycle regulation governs cell growth and division.
Studying cell cycle progression requires synchronized cell populations.
Various synchronization methods exist, each with pros and cons.

Purpose of the Study:

To describe protocols for large-scale isolation of mitotic mammalian cells.
To detail methods for enriching cells at specific mitotic stages.
To outline optimized culture conditions for cell cycle studies.

Main Methods:

Utilizing drug-dependent synchronization for mitotic enrichment.
Implementing techniques for adherent and suspension cell isolation.
Optimizing culture conditions for specific mitotic stage enrichment.

More Related Videos

Studying Cell Cycle-regulated Gene Expression by Two Complementary Cell Synchronization Protocols

Studying Cell Cycle-regulated Gene Expression by Two Complementary Cell Synchronization Protocols

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Mammalian Cell Division in 3D Matrices via Quantitative Confocal Reflection Microscopy

Mammalian Cell Division in 3D Matrices via Quantitative Confocal Reflection Microscopy

Published on: November 29, 2017

Related Experiment Videos

Last Updated: May 31, 2026

Combining Mitotic Cell Synchronization and High Resolution Confocal Microscopy to Study the Role of Multifunctional Cell Cycle Proteins During Mitosis

Combining Mitotic Cell Synchronization and High Resolution Confocal Microscopy to Study the Role of Multifunctional Cell Cycle Proteins During Mitosis

Published on: December 5, 2017

Studying Cell Cycle-regulated Gene Expression by Two Complementary Cell Synchronization Protocols

Studying Cell Cycle-regulated Gene Expression by Two Complementary Cell Synchronization Protocols

Published on: June 6, 2017

Mammalian Cell Division in 3D Matrices via Quantitative Confocal Reflection Microscopy

Mammalian Cell Division in 3D Matrices via Quantitative Confocal Reflection Microscopy

Published on: November 29, 2017

Main Results:

Established protocols for large-scale mitotic mammalian cell isolation.
Demonstrated methods for enriching cells at distinct mitotic phases.
Provided optimized culture conditions for selected mitotic stages.

Conclusions:

Drug-dependent synchronization is advantageous for studying later cell cycle events.
The described protocols facilitate large-scale isolation of mitotic cells.
Optimized methods ensure specific enrichment at desired mitotic stages for research.