Accuracy of GFR predictive equations using ID-MS traceable creatinine: compensated Jaffe and enzymatic
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Published on: May 10, 2013
Anne-Sophie Bargnoux1, Noémie Simon, Valérie Garrigue
1Department of Biochemistry, University of Montpellier 1, Montpellier, France.
Background:
New ID-MS traceable creatinine kits including enzymatic creatinine (e-creat) and compensated Jaffe (j-creat) has been developed by Olympus.
Methods:
Analytical performances of these reagents were tested on an AU 640 analyzer. A comparison study was performed against the method used in our laboratory (Randox creatinine enzymatic). Values of the glomerular filtration rate estimated (eGFR) from ID-MS traceable equation were compared to the GFR measured (mGFR) by a reference isotope method in a kidney transplant cohort.
Results:
Total intra- and inter-assay variation coefficients were below 4%. Creatinine values were correlated with the Randox enzymatic method (e-creat = 0.98 Randox - 0.48 and j-creat = 0.99 Randox - 1.44, r2 = 0.98). Values of eGFR obtained with both e-creat and j-creat were well correlated to mGFR in our population of renal transplants.
Conclusions:
The new ID-MS traceable methods improved estimation of GFR by predictive equations. However, the limitation of the Jaffe method in specific populations such as paediatric and elderly patients needs further investigations.
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