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Related Experiment Video

Updated: May 31, 2026

Assessing Autophagic Flux by Measuring LC3, p62, and LAMP1 Co-localization Using Multispectral Imaging Flow Cytometry
11:39

Assessing Autophagic Flux by Measuring LC3, p62, and LAMP1 Co-localization Using Multispectral Imaging Flow Cytometry

Published on: July 21, 2017

Spectral imaging-based methods for quantifying autophagy and apoptosis.

Nathan G Dolloff1, Xiahong Ma, David T Dicker

  • 1Laboratory of Translational Oncology and Experimental Cancer Therapeutics, Department of Medicine, Penn State College of Medicine, Penn State Hershey Cancer Institute, Penn State Hershey Medical Center, PA, USA.

Cancer Biology & Therapy
|July 16, 2011
PubMed
Summary
This summary is machine-generated.

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Spectral imaging quantifies autophagy and apoptosis by analyzing light signatures. This method accurately measures autophagosome formation and cell death, offering a novel approach for biological research.

Area of Science:

  • Biophotonics and Imaging
  • Cellular Biology
  • Molecular Pathology

Background:

  • Spectral imaging detects subtle light differences, enabling quantitative analysis.
  • Autophagy and apoptosis are critical cellular processes requiring precise quantification.
  • Existing methods for studying these processes have limitations.

Purpose of the Study:

  • To develop and validate spectral imaging methods for quantifying autophagy and apoptosis.
  • To establish quantitative assays for autophagosome formation using LC3-GFP.
  • To identify spectral signatures for apoptosis in tumor samples and assess drug efficacy.

Main Methods:

  • Coupling spectral imaging with fluorescence and white light microscopy.
  • Utilizing multispectral imaging to analyze spectral changes in LC3-GFP fluorescence.

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Quantitative Analysis of Autophagy using Advanced 3D Fluorescence Microscopy
09:59

Quantitative Analysis of Autophagy using Advanced 3D Fluorescence Microscopy

Published on: May 3, 2013

Related Experiment Videos

Last Updated: May 31, 2026

Assessing Autophagic Flux by Measuring LC3, p62, and LAMP1 Co-localization Using Multispectral Imaging Flow Cytometry
11:39

Assessing Autophagic Flux by Measuring LC3, p62, and LAMP1 Co-localization Using Multispectral Imaging Flow Cytometry

Published on: July 21, 2017

Quantitative Analysis of Autophagy using Advanced 3D Fluorescence Microscopy
09:59

Quantitative Analysis of Autophagy using Advanced 3D Fluorescence Microscopy

Published on: May 3, 2013

  • Employing the Prism and Reflector Imaging Spectroscopy System (PARISS) for hyperspectral imaging of apoptosis.

    • Main Results:

    • Distinct spectral profiles differentiated punctate (autophagosome-associated) from diffuse (cytosolic) LC3-GFP.
    • Hydroxychloroquine treatment significantly altered the LC3-GFP spectral signature, confirming the autophagy assay.
    • PARISS identified a spectral signature for active caspase-8 and quantified apoptosis in tumor samples, even in H&E-stained sections.

    Conclusions:

    • Spectral imaging offers an unbiased, quantitative, and rapid method for studying autophagy.
    • PARISS enables accurate quantification of apoptosis, including death receptor-mediated pathways.
    • These spectral imaging techniques complement existing methodologies in cell death and autophagy research.