Jove
Visualize
Contact Us

Related Concept Videos

Phase Contrast and Differential Interference Contrast Microscopy01:26

Phase Contrast and Differential Interference Contrast Microscopy

Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
Three-Dimensional Microscopy in Microbiology01:28

Three-Dimensional Microscopy in Microbiology

Three-dimensional imaging techniques are essential in cell biology, allowing researchers to visualize intricate cellular structures with high resolution. Two prominent methods, Differential Interference Contrast Microscopy (DIC) and Confocal Scanning Laser Microscopy (CSLM), provide distinct advantages for imaging live and thick specimens, respectively.Differential Interference Contrast MicroscopyDIC microscopy enhances contrast in transparent, unstained samples by converting phase...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Facilitation of macrofaunal assemblages in marinas by the habitat-forming invader Amathia verticillata (Bryozoa: Gymnolaemata) across a spatiotemporal scale.

Marine environmental research·2023
Same author

Outcomes and complications after phacoemulsification in retinoblastoma patients with cataract after radiation treatment.

Archivos de la Sociedad Espanola de Oftalmologia·2016
Same author

Illumina next generation sequencing data and expression microarrays data from retinoblastoma and medulloblastoma tissues.

Data in brief·2016
Same author

Overview of recurrent chromosomal losses in retinoblastoma detected by low coverage next generation sequencing.

Cancer genetics·2016
Same author

Retinopathy of prematurity as a major cause of severe visual impairment and blindness in children in schools for the blind in Guadalajara city, Mexico.

The British journal of ophthalmology·2011
Same author

Clinical delineation of a patient with trisomy 1q32.qter and monosomy 5p resulting from a familial translocation 1;5.

Genetic counseling (Geneva, Switzerland)·2011
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: May 31, 2026

Isolation of Cells with Morphological and Spatial Information from Oral Submucous Fibrosis Samples by Laser Capture Microdissection
05:42

Isolation of Cells with Morphological and Spatial Information from Oral Submucous Fibrosis Samples by Laser Capture Microdissection

Published on: August 11, 2023

Temporary morphological changes in plus disease induced during contact digital imaging.

L C Zepeda-Romero1, M E Martinez-Perez, S Ruiz-Velasco

  • 1Retinopathy of Prematurity Clinic, O.P.D. Hospital Civil de Guadalajara, Centro Universitario de Ciencias de la Salud, Universidad de Guadalajara, Guadalajara, Mexico. drconsuelo@yahoo.com

Eye (London, England)
|July 16, 2011
PubMed
Summary

Digital camera pressure during retinopathy of prematurity (ROP) imaging can alter retinal vessels. Careful technique is crucial to prevent vessel compression and ensure accurate ROP diagnosis.

More Related Videos

In Vivo Dynamics of Retinal Microglial Activation During Neurodegeneration: Confocal Ophthalmoscopic Imaging and Cell Morphometry in Mouse Glaucoma
12:48

In Vivo Dynamics of Retinal Microglial Activation During Neurodegeneration: Confocal Ophthalmoscopic Imaging and Cell Morphometry in Mouse Glaucoma

Published on: May 11, 2015

Microtubule Plus-End Dynamics Visualization in Huntington's Disease Model based on Human Primary Skin Fibroblasts
10:38

Microtubule Plus-End Dynamics Visualization in Huntington's Disease Model based on Human Primary Skin Fibroblasts

Published on: January 8, 2022

Related Experiment Videos

Last Updated: May 31, 2026

Isolation of Cells with Morphological and Spatial Information from Oral Submucous Fibrosis Samples by Laser Capture Microdissection
05:42

Isolation of Cells with Morphological and Spatial Information from Oral Submucous Fibrosis Samples by Laser Capture Microdissection

Published on: August 11, 2023

In Vivo Dynamics of Retinal Microglial Activation During Neurodegeneration: Confocal Ophthalmoscopic Imaging and Cell Morphometry in Mouse Glaucoma
12:48

In Vivo Dynamics of Retinal Microglial Activation During Neurodegeneration: Confocal Ophthalmoscopic Imaging and Cell Morphometry in Mouse Glaucoma

Published on: May 11, 2015

Microtubule Plus-End Dynamics Visualization in Huntington's Disease Model based on Human Primary Skin Fibroblasts
10:38

Microtubule Plus-End Dynamics Visualization in Huntington's Disease Model based on Human Primary Skin Fibroblasts

Published on: January 8, 2022

Area of Science:

  • Ophthalmology
  • Medical Imaging
  • Computational Analysis

Background:

  • Retinopathy of prematurity (ROP) requires regular imaging for monitoring.
  • Handheld retinal cameras can inadvertently cause pressure artifacts during imaging.
  • Quantifying these artifacts is essential for diagnostic accuracy.

Purpose of the Study:

  • To quantify retinal vascular changes caused by camera pressure during ROP imaging.
  • To compare vascular parameters between images with and without compression artifacts.
  • To assess the impact of pressure on retinal vessel diameter, curvature, and tortuosity.

Main Methods:

  • Analysis of wide-angle retinal photographs from routine ROP examinations.
  • Matching vascular trees in images with and without compression artifacts.
  • Utilizing Retinal Image multiScale Analysis (RIMA) software for quantitative assessment.
  • Statistical analysis using a two-level linear model for integrated curvature (IC), diameter (d), and tortuosity index (TI).

Main Results:

  • Significant reduction in arteriolar and venular diameter (d) in images with compression artifacts (P<0.01).
  • Significant decrease in arterial integrated curvature (IC) in compressed images (P<0.05).
  • No significant changes in vascular tortuosity index (TI) were observed between groups.

Conclusions:

  • Non-intentional corneal pressure from the RetCam lens can compress and alter retinal vessel diameter.
  • Severe pressure may lead to the collapse of retinal vessels.
  • Avoiding compression artifacts, indicated by the absence of the arterial blood column at the optic nerve head, is critical for accurate ROP imaging.