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Related Concept Videos

Ribosome Profiling02:24

Ribosome Profiling

Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique helps...

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Related Experiment Video

Updated: May 31, 2026

Cerebrospinal Fluid MicroRNA Profiling Using Quantitative Real Time PCR
09:26

Cerebrospinal Fluid MicroRNA Profiling Using Quantitative Real Time PCR

Published on: January 22, 2014

MicroRNA profiling using RNA from microdissected immunostained tissue.

Clemens L Bockmeyer1, Danny Jonigk, Hans Kreipe

  • 1Institute of Pathology, Medizinische Hochschule Hannover, Hannover, Germany.

Methods in Molecular Biology (Clifton, N.J.)
|July 16, 2011
PubMed
Summary
This summary is machine-generated.

Researchers identified distinct microRNA profiles in healthy luminal and basal breast epithelial cells. This was achieved by developing a new method to isolate these specific cell types from tissue samples for analysis.

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miRNA Expression Analyses in Prostate Cancer Clinical Tissues
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miRNA Expression Analyses in Prostate Cancer Clinical Tissues

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Last Updated: May 31, 2026

Cerebrospinal Fluid MicroRNA Profiling Using Quantitative Real Time PCR
09:26

Cerebrospinal Fluid MicroRNA Profiling Using Quantitative Real Time PCR

Published on: January 22, 2014

High Throughput MicroRNA Profiling: Optimized Multiplex qRT-PCR at Nanoliter Scale on the Fluidigm Dynamic ArrayTM IFCs
07:27

High Throughput MicroRNA Profiling: Optimized Multiplex qRT-PCR at Nanoliter Scale on the Fluidigm Dynamic ArrayTM IFCs

Published on: August 3, 2011

miRNA Expression Analyses in Prostate Cancer Clinical Tissues
11:29

miRNA Expression Analyses in Prostate Cancer Clinical Tissues

Published on: September 8, 2015

Area of Science:

  • Molecular Biology
  • Oncology
  • Genomics

Background:

  • Messenger RNA (mRNA) expression profiling defines human breast cancer molecular subtypes.
  • MicroRNAs (miRNAs) are implicated in breast cancer subtypes, but their role in healthy epithelial cells is unclear.
  • Limited understanding exists regarding the specific miRNA signatures of normal luminal and basal breast epithelial cells.

Purpose of the Study:

  • To characterize the distinct microRNA profiles of healthy luminal and basal breast epithelial cells.
  • To establish a novel method for isolating specific breast epithelial cell populations from fixed tissues.

Main Methods:

  • Development of a laser microdissection technique to isolate immunostained luminal and basal breast epithelial cells.
  • Utilized formalin-fixed paraffin-embedded (FFPE) tissues for cell isolation.
  • Employed real-time PCR-based profiling for microRNA analysis.

Main Results:

  • Successfully isolated distinct luminal and basal breast epithelial cell populations using the developed method.
  • Identified unique microRNA signatures specific to luminal breast epithelial cells.
  • Identified unique microRNA signatures specific to basal breast epithelial cells.

Conclusions:

  • The study successfully established a method for isolating and profiling miRNAs from specific healthy breast epithelial cell types.
  • Distinct microRNA profiles were identified for luminal and basal breast epithelial cells, providing a baseline for future cancer research.
  • This research lays the groundwork for understanding miRNA roles in early breast tissue and potential diagnostic applications.