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Updated: May 31, 2026
A Generalized Method for Determining Free Soluble Phenolic Acid Composition and Antioxidant Capacity of Cereals and Legumes
Published on: June 10, 2022
[Fast determination of phenolic acids in Lycium ruthenicum murr juice by solid phase extraction and HPLC]
Chen Chen1, Huaixiu Wen, Xiaohui Zhao
1Northwest Institute of Plateau Biology Chinese Academy of Sciences, Xining 810008, China. chenchen19841014@163.com
Objective:
To establish a quantitative method for determination of gallic acid, original catechins, catechins, chlorogenic acid, caffeic acid, syringic acid in Lycium ruthenicum.
Method:
The sample was separated on an ODS column (4. 6 mm x 250 mm, 5 pnm), eluted with methanol and water containing 0. 1% acid with detected wavelength at 280 nm, and flow rate at 1. 0 mL x min(-1).
Result:
The linearity of six components were good (r = 0.9999). The average recoveries (n=5) of the six constituents were 97. 70% (RSD 2.3%), 99.64% (RSD 1.8%), 100.7% (RSD 2.1%), 99.98% (RSD 2.6%), 99.60% (RSD 2.2%), 99.04% (RSD 2.4%), respectively.
Conclusion:
The method is rapid and precise. It can be used for quality control of Lycium ruthenicum juice.
