Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Structural features of a regulatory nucleosome.

B Piña1, D Barettino, M Truss

  • 1Institut für Molekularbiologie und Tumorforschung, Marburg, F.R.G.

Journal of Molecular Biology
|December 20, 1990
PubMed
Summary

Mouse mammary tumor virus (MMTV) long terminal repeat (LTR) DNA sequences intrinsically position nucleosomes. DNA bendability and sequence features dictate histone octamer placement, supporting a modular model for nucleosome phasing.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

<i>In vivo</i> motor unit decoding and <i>in vitro</i> cellular characterisation of spinal circuits for urination in adult mice.

bioRxiv : the preprint server for biology·2026
Same author

[In memory of Professor Dr. med. Dr. h.c. Jürgen Sökeland : * 25 September 1933 † 27 November 2023].

Urologie (Heidelberg, Germany)·2024
Same author

Multifocal rapidly involuting congenital haemangiomas.

Journal of the European Academy of Dermatology and Venereology : JEADV·2023
Same author

Occurrence of antibiotics in Lettuce (Lactuca sativa L.) and Radish (Raphanus sativus L.) following organic soil fertilisation under plot-scale conditions: Crop and human health implications.

Journal of hazardous materials·2022
Same author

Prevalence of mucocutaneous manifestations in 666 patients with COVID-19 in a field hospital in Spain: oral and palmoplantar findings.

The British journal of dermatology·2020
Same author

Viral respiratory tract infections in the neonatal intensive care unit.

Anales de pediatria·2020

Area of Science:

  • Molecular Biology
  • Epigenetics
  • Structural Biology

Background:

  • Nucleosomes are fundamental units of chromatin, influencing DNA accessibility and gene regulation.
  • The mouse mammary tumor virus long terminal repeat (MMTV-LTR) is known to interact with histone octamers.

Purpose of the Study:

  • To investigate the DNA sequence determinants of nucleosome positioning on the MMTV-LTR.
  • To elucidate the mechanisms underlying both rotational and translational positioning of nucleosomes.

Main Methods:

  • Chromatin reconstitution experiments using MMTV-LTR DNA fragments.
  • Analysis of nucleosome positioning in vitro on linear and circular DNA molecules.
  • Assessment of DNA bendability and sequence-specific effects on histone octamer binding.

Related Experiment Videos

Main Results:

  • MMTV-LTR DNA sequences (-236 to +204) accommodate two histone octamers in positions consistent with in vivo data.
  • Nucleosome positioning is independent of DNA fragment length and occurs on both linear and circular DNA.
  • Intrinsic DNA bendability of MMTV-LTR correlates with rotational nucleosome positioning; sequence features influence translational positioning.

Conclusions:

  • Intrinsic DNA bendability is a key factor in rotational nucleosome positioning on MMTV-LTR.
  • A modular model, based on additive sequence features, explains the observed nucleosome phasing.
  • Understanding these mechanisms provides insights into epigenetic regulation by viral LTRs.