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Related Experiment Video

Updated: May 31, 2026

Imaging of HIV-1 Envelope-induced Virological Synapse and Signaling on Synthetic Lipid Bilayers

Imaging of HIV-1 Envelope-induced Virological Synapse and Signaling on Synthetic Lipid Bilayers

Published on: March 8, 2012

Visualizing HIV-1 assembly.

Nolwenn Jouvenet¹, Sanford M Simon, Paul D Bieniasz

¹Laboratory of Retrovirology, The Rockefeller University, New York, NY 10016, USA.

Journal of Molecular Biology

|July 19, 2011

Summary

Live-cell imaging reveals the dynamics of HIV-1 particle assembly. Single-virion techniques illuminate Gag protein assembly, RNA packaging, and ESCRT protein recruitment in real-time.

Area of Science:

Virology
Cell Biology
Microscopy

Background:

HIV-1 particle assembly is a complex process involving viral and cellular components.
Traditional macroscopic and fixed-cell microscopy offer limited insights into dynamic assembly mechanisms.

Purpose of the Study:

To review how live-cell microscopic imaging advances the understanding of HIV-1 particle assembly.
To highlight findings from single-virion imaging techniques in live cells.

Main Methods:

Live-cell microscopic imaging techniques.
Total internal reflection fluorescence microscopy (TIR-FM).
Single-virion imaging in live cells.

Main Results:

Illuminated dynamics of Gag protein assembly at the individual particle level.

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Structure of HIV-1 Capsid Assemblies by Cryo-electron Microscopy and Iterative Helical Real-space Reconstruction

Structure of HIV-1 Capsid Assemblies by Cryo-electron Microscopy and Iterative Helical Real-space Reconstruction

Published on: August 9, 2011

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Last Updated: May 31, 2026

Imaging of HIV-1 Envelope-induced Virological Synapse and Signaling on Synthetic Lipid Bilayers

Imaging of HIV-1 Envelope-induced Virological Synapse and Signaling on Synthetic Lipid Bilayers

Published on: March 8, 2012

Structure of HIV-1 Capsid Assemblies by Cryo-electron Microscopy and Iterative Helical Real-space Reconstruction

Structure of HIV-1 Capsid Assemblies by Cryo-electron Microscopy and Iterative Helical Real-space Reconstruction

Published on: August 9, 2011

Revealed mechanisms of viral RNA packaging in real-time.

Detailed the recruitment of Endosomal Sorting Complex Required for Transport (ESCRT) proteins during assembly.

Conclusions:

Individual particle imaging in living cells overcomes limitations of traditional methods.
Live-cell microscopy provides crucial insights into HIV-1 assembly dynamics.
This approach yields findings unattainable with macroscopic or fixed-cell techniques.