Related Experiment Video
Updated: May 31, 2026
06:06
In Vitro Analysis of E3 Ubiquitin Ligase Function
Published on: May 14, 2021
Notch ligand ubiquitylation: what is it good for?
Gerry Weinmaster1, Janice A Fischer
1Department of Biological Chemistry, David Geffen School of Medicine, University of California-Los Angeles, CA 90095, USA.
Developmental Cell
|July 19, 2011
Summary
The gene neuralized in Drosophila encodes a ubiquitin ligase that targets the Notch ligand Delta for endocytosis. This process, unique to Notch signaling, is crucial for signal transmission.
Area of Science:
- Developmental biology
- Cell signaling
- Molecular genetics
Background:
- The neurogenic gene neuralized in Drosophila encodes a ubiquitin ligase.
- This ligase monoubiquitylates the Notch ligand Delta, promoting its endocytosis.
- Ligand internalization by the signal-sending cell is a unique feature of Notch signaling.
Purpose of the Study:
- To review the development of ideas regarding Notch signaling.
- To discuss the role of ligand ubiquitylation and endocytosis in Notch signaling.
Main Methods:
- Literature review and discussion of previous findings.
Main Results:
- Ubiquitylation and endocytosis of the Notch ligand Delta are essential for Notch signaling.
- The mechanism of ligand internalization by the signal-sending cell is a conserved feature.
Conclusions:
- Ubiquitylation and endocytosis of Delta are key regulators of Notch signaling.
- Further research is needed to fully elucidate the mechanisms involved.
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The Notch signaling pathway is a major intracellular signaling pathway that is highly conserved over a broad spectrum of metazoan species. It stands unique from other intracellular signaling mechanisms in animals because notch protein itself acts as the receptor as well as the primary signaling molecule.
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not until 1985...
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not until 1985...
03:14Notch Signaling Pathway
The Notch signaling pathway is a major intracellular signaling pathway that is highly conserved over a broad spectrum of metazoan species. It stands unique from other intracellular signaling mechanisms in animals because notch protein itself acts as the receptor as well as the primary signaling molecule.
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not until 1985...
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not until 1985...
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These groups modify specific amino acids in a protein.
These groups modify specific amino acids in a protein.
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