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A High-throughput Platform for the Screening of Salmonella spp./Shigella spp.
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Evaluation of the colonization capabilities of Salmonella Enteritidis in quails using an RT-PCR approach

Guang-Zhi He1, Yong Feng, Shu-Xuan Deng

  • 1Guiyang College of Traditional Chinese Medicine, Guiyang 550002, Guizhou Province, China.

Research in Veterinary Science
|July 19, 2011
PubMed

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Real Time RT-PCR02:57

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...