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Related Concept Videos

RNA-seq03:21

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while microarray-based...
Next-generation Sequencing03:00

Next-generation Sequencing

The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features.
Sanger Sequencing01:57

Sanger Sequencing

DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...

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Related Experiment Video

Updated: May 31, 2026

Next-generation Sequencing of 16S Ribosomal RNA Gene Amplicons
10:24

Next-generation Sequencing of 16S Ribosomal RNA Gene Amplicons

Published on: August 29, 2014

Strategy for modular tagged high-throughput amplicon sequencing.

Daniel Aguirre de Cárcer1, Stuart E Denman, Chris McSweeney

  • 1CSIRO Preventative Health National Research Flagship and Livestock Industries, Queensland Bioscience Precinct, St. Lucia, QLD 4067, Australia.

Applied and Environmental Microbiology
|July 19, 2011
PubMed
Summary
This summary is machine-generated.

A new strategy enables universal bar-coded sequencing primers to attach to amplified PCR products. This modular approach allows the same barcode to be used with multiple primer sets, even at the same time.

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Last Updated: May 31, 2026

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Area of Science:

  • Molecular Biology
  • Genetics
  • Biotechnology

Background:

  • Polymerase Chain Reaction (PCR) amplification is a cornerstone of molecular biology.
  • Sequencing DNA requires specific adapters and primers for efficient amplification and read generation.
  • Current methods can be cumbersome, requiring unique adapters for different target-specific primers.

Purpose of the Study:

  • To describe and validate a novel strategy for appending universal bar-coded sequencing primers.
  • To enable a modular approach to DNA sequencing library preparation.
  • To facilitate the simultaneous use of multiple target-specific primers with a single barcode.

Main Methods:

  • Development of a strategy for universal primer attachment to PCR products.
  • Validation of the bar-coded primer strategy.
  • Demonstration of modularity with multiple target-specific primer sets.

Main Results:

  • Successful implementation of a strategy for universal bar-coded primer appending.
  • Demonstrated modularity, allowing a single barcode to be used with diverse primer sets.
  • Validation of the approach for simultaneous use of multiple primer sets.

Conclusions:

  • The described strategy offers a flexible and efficient method for preparing sequencing libraries.
  • This modular bar-coding approach simplifies workflows and potentially reduces costs in DNA sequencing.
  • The method supports simultaneous multiplexing of target-specific amplification.