Related Experiment Video

Updated: May 31, 2026

An Adoptive Transfer Model of Rheumatoid Arthritis in Mice
07:37

An Adoptive Transfer Model of Rheumatoid Arthritis in Mice

Published on: June 6, 2025

Tyk2 deficiency protects joints against destruction in anti-type II collagen antibody-induced arthritis in mice

Masayuki Ishizaki1, Ryuta Muromoto, Toshihiko Akimoto

  • 1Department of Immunology, Graduate School of Pharmaceutical Sciences, Hokkaido University, Sapporo, Japan.

International Immunology
|July 19, 2011
PubMed

Insights

Tyrosine kinase-2 (Tyk2) is crucial for arthritis development. Tyk2-deficient mice showed no arthritis symptoms, indicating Tyk2

Area of Science:

  • Immunology
  • Rheumatology

Background:

  • Tyrosine kinase-2 (Tyk2) is involved in cytokine signaling in immune responses.
  • Cytokine signaling plays a role in the pathogenesis of autoimmune diseases like arthritis.

Purpose of the Study:

  • To investigate the role of Tyk2 in anti-type II collagen antibody-induced arthritis (CAIA) in vivo.
  • To determine if Tyk2 deficiency protects against arthritis development.

Main Methods:

  • Utilized Tyk2-deficient mice and wild-type littermates.
  • Induced arthritis using anti-type II collagen antibodies (CAIA model).
  • Assessed clinical signs of arthritis, inflammatory cell infiltration, cartilage fibrillation, and cytokine/matrix metalloprotease production.

Main Results:

  • Wild-type mice developed severe arthritis with swelling and erythema.
  • Tyk2-deficient mice exhibited no signs of arthritis, inflammation, or cartilage damage.
  • Tyk2 deficiency significantly reduced pro-inflammatory cytokines (Th1/Th17) and matrix metalloproteases in the affected paws.

Conclusions:

  • Tyk2 plays a critical role in the development of CAIA.
  • Tyk2 deficiency confers protection against arthritis.
  • Tyk2 is a potential therapeutic target for drug development in arthritis treatment.

More Related Videos

Development of Stem Cell-derived Antigen-specific Regulatory T Cells Against Autoimmunity10:10

Development of Stem Cell-derived Antigen-specific Regulatory T Cells Against Autoimmunity

Published on: November 8, 2016

A Cryo-pulverization Protocol for Processing Mouse Paws to Evaluate Molecular Pathways of Tissue Inflammation in a Collagen Induced Arthritis Model11:03

A Cryo-pulverization Protocol for Processing Mouse Paws to Evaluate Molecular Pathways of Tissue Inflammation in a Collagen Induced Arthritis Model

Published on: October 30, 2019

Related Concept Videos

T Cell Types and Functions01:24

T Cell Types and Functions

When T cells with CD4 markers are activated, they give rise to two types of effector cells: helper T cells and regulatory T cells. Meanwhile, T cells with CD8 markers differentiate into effector cytotoxic T cells. The differentiation of CD4 T cells into helper T cell subsets, such as Th1, Th2, and Th17 cells, is dependent on the antigen type, antigen-presenting cell, and regulatory cytokines.
Th1 cells stimulate dendritic cells to express necessary co-stimulatory molecules on their surfaces for...
The JAK-STAT Signaling Pathway01:20

The JAK-STAT Signaling Pathway

Several cytokine receptors have tightly bound Janus kinase or JAK proteins attached at their cytosolic tail. Small signaling molecules such as cytokines, growth hormones, or prolactins bind to the cytokine receptors and initiate their dimerization. The dimerization brings the cytosolic JAKs together that trans-phosphorylate and activates each other. The activated JAKs now phosphorylate cytosolic tails of the cytokine receptors, which serve as binding sites for adaptor proteins such as  SH2...