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Updated: May 31, 2026

Light-sheet Fluorescence Microscopy for the Study of the Murine Heart
Published on: September 15, 2018
Deep and fast live imaging with two-photon scanned light-sheet microscopy
Thai V Truong1, Willy Supatto, David S Koos
1California Institute of Technology, Beckman Institute, Pasadena, CA, USA.tvt@caltech.edu
Abstract:
We implemented two-photon scanned light-sheet microscopy, combining nonlinear excitation with orthogonal illumination of light-sheet microscopy, and showed its excellent performance for in vivo, cellular-resolution, three-dimensional imaging of large biological samples. Live imaging of fruit fly and zebrafish embryos confirmed that the technique can be used to image up to twice deeper than with one-photon light-sheet microscopy and more than ten times faster than with point-scanning two-photon microscopy without compromising normal biology.
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