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Updated: May 31, 2026
09:11
Genome Editing with CompoZr Custom Zinc Finger Nucleases (ZFNs)
Published on: June 14, 2012
High-frequency genome editing using ssDNA oligonucleotides with zinc-finger nucleases
Fuqiang Chen1, Shondra M Pruett-Miller, Yuping Huang
1Sigma-Aldrich Biotechnology, St. Louis, Missouri, USA.
Nature Methods
|July 19, 2011
Abstract
None:
Zinc-finger nucleases (ZFNs) have enabled highly efficient gene targeting in multiple cell types and organisms. Here we describe methods for using simple ssDNA oligonucleotides in tandem with ZFNs to efficiently produce human cell lines with three distinct genetic outcomes: (i) targeted point mutation, (ii) targeted genomic deletion of up to 100 kb and (iii) targeted insertion of small genetic elements concomitant with large genomic deletions.
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