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Updated: May 31, 2026
Proton Transfer and Protein Conformation Dynamics in Photosensitive Proteins by Time-resolved Step-scan Fourier-transform Infrared Spectroscopy
Published on: June 27, 2014
Photoionization of a protein isolated in vacuo
Aleksandar R Milosavljević1, Christophe Nicolas, Joel Lemaire
1Synchrotron SOLEIL, L'Orme des Merisiers, Saint Aubin, B.P. 48, 91192 Gif-sur-Yvette, France. vraz@ipb.ac.rs
Abstract:
Gas phase VUV single-photon photoionization spectroscopy of electrospray-produced multiply protonated cytochrome c protein (12 kDa) has been performed by means of coupling a linear quadrupole ion trap with a synchrotron beamline. The thresholds for the ionization of the 8+ and 11+ charge state precursors to radical 9+ and 12+ species were measured to be 12.75 ± 0.10 and 13.51 ± 0.10 eV, respectively.
