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Predictive performance for human skin sensitizing potential of the human cell line activation test (h-CLAT)
Yuko Nukada1, Takao Ashikaga, Hitoshi Sakaguchi
1Kao Corporation, Safety Science Research Laboratories, Tochigi 321-3497, Japan. nukada.yuko@kao.co.jp
Background:
Recent changes in regulatory restrictions and social opposition to animal toxicology experiments have driven the need for reliable in vitro tests for predicting the skin sensitizing potentials of a wide variety of industrial chemicals. Previously, we developed the human cell line activation test (h-CLAT) as a cell-based assay to predict the skin sensitizing potential of chemicals, and showed the correspondence between the h-CLAT and the murine local lymph node assay results.
Objectives:
This study was conducted to investigate the predictive performance of the h-CLAT for human skin sensitizing potential.
Materials/Methods:
We selected a total of 66 test chemicals with known human sensitizing potential, and tested all chemicals with the h-CLAT. We then evaluated the performance of the h-CLAT in predicting human sensitizing potential.
Results And Conclusion:
Forty-five of 51 tested sensitizers were positive in the h-CLAT, indicating relatively high sensitivity. Also, 10 of 15 non-sensitizers were correctly detected as negative. The overall agreement between human data and h-CLAT outcome was 83%. Furthermore, the h-CLAT could accurately predict the human sensitizing potential of 23 tested chemicals that were amines, heterocyclic compounds, or sulfur compounds. Our data indicate the utility of the h-CLAT for predicting the human skin sensitizing potential of a variety of chemicals.
