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Role for the Burkholderia pseudomallei type three secretion system cluster 1 bpscN gene in virulence

Tanya D'Cruze1, Lan Gong, Puthayalai Treerat

  • 1Department of Biochemistry and Molecular Biology, Monash University, Clayton Campus, VIC 3800, Australia.

Infection and Immunity
|July 20, 2011
PubMed

Insights

The type three secretion system 1 (TTSS1) in Burkholderia pseudomallei is crucial for virulence. A TTSS1 mutant showed reduced survival in host cells and attenuated infection in mice, highlighting its role in melioidosis pathogenesis.

Area of Science:

  • Microbiology
  • Pathogenesis
  • Bacterial Virulence Factors

Background:

  • Burkholderia pseudomallei causes melioidosis, a serious infection.
  • Type three secretion systems (TTSS) deliver bacterial effectors into host cells.
  • B. pseudomallei possesses three TTSS gene clusters (TTSS1, TTSS2, TTSS3).

Purpose of the Study:

  • To investigate the role of TTSS1, specifically the bpscN gene product, in B. pseudomallei virulence.
  • To characterize a B. pseudomallei mutant lacking TTSS1 ATPase expression.

Main Methods:

  • Respiratory infection model in mice.
  • In vitro studies using RAW264.7 macrophage cell line.
  • Analysis of bacterial survival, replication, phagosomal escape, and autophagy colocalization.

Main Results:

  • A TTSS1 bpscN mutant was significantly attenuated in a murine respiratory melioidosis model.
  • The mutant exhibited diminished survival and replication within RAW264.7 cells.
  • Increased colocalization with the autophagy marker LC3 was observed in the mutant.

Conclusions:

  • The TTSS1 bpscN gene product is essential for intracellular survival of B. pseudomallei.
  • TTSS1 plays a significant role in the pathogenesis of murine melioidosis.

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