Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Two-dimensional Gel Electrophoresis01:22

Two-dimensional Gel Electrophoresis

Two-dimensional gel electrophoresis is a high-resolution protein separation method first introduced by O' Farrell and Klose in 1975. This method involves protein separation by two dimensions, mass and charge, making it more accurate than one-dimensional gel electrophoresis.
The first dimension separation uses the isoelectric focusing or IEF technique performed on immobilized pH gradient (IPG) strips that separate proteins according to their isoelectric points.
Biological samples, such as  cells...
Electrophoresis: Overview01:20

Electrophoresis: Overview

Electrophoresis is a powerful analytical separation technique that relies on the differential migration of charged species when subjected to an electric field. The core strength of electrophoresis lies in its ability to separate high-molecular-weight species in complex mixtures. It has found widespread use in biochemistry, molecular biology, and analytical chemistry, allowing the separation of compounds like amino acids, nucleotides, carbohydrates, and proteins with excellent resolution.
There...
Capillary Electrophoresis: Instrumentation01:20

Capillary Electrophoresis: Instrumentation

Capillary electrophoresis instrumentation typically consists of several key components. A high-voltage power supply generates the electric field necessary for the separation by connecting to an anode (the positively charged electrode) and a cathode (the negatively charged electrode) located in buffer reservoirs at each end of the capillary tube. The system includes a sample vial, a fused silica capillary tube coated with polyimide for mechanical strength through which the sample components...
Capillary Electrophoresis: Applications01:30

Capillary Electrophoresis: Applications

Capillary electrophoretic separations offer various modes, each with unique applications. These modes include capillary zone electrophoresis, capillary gel electrophoresis, capillary array electrophoresis, capillary isoelectric focusing, capillary isotachophoresis, micellar electrokinetic chromatography, and capillary electrochromatography.
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...
DNA Agarose Gel Electrophoresis02:35

DNA Agarose Gel Electrophoresis

Agarose gel electrophoresis is a laboratory technique commonly used to separate DNA fragments by size. However, it can also be used to isolate and purify DNA fragments using a gel extraction protocol.
Gel extraction follows five major steps: running gel electrophoresis to separate fragments, isolating the individual bands, extracting DNA from those bands, and removing the dye and salts from the extracted mixture to obtain pure DNA.
In cloning experiments, both the insert and vector DNA...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Dishevelled‑2 modulates osteogenic differentiation of human synovial fibroblasts in osteoarthritis.

Molecular medicine reports·2018
Same author

Effects of intestinal colonization by Clostridium difficile and Staphylococcus aureus on microbiota diversity in healthy individuals in China.

BMC infectious diseases·2018
Same author

An ultrasonic nanobubble-mediated PNP/fludarabine suicide gene system: A new approach for the treatment of hepatocellular carcinoma.

PloS one·2018
Same author

An integrative approach to investigate the association among high-sensitive C-reactive protein, body fat mass distribution, and other cardiometabolic risk factors in young healthy women.

Methods (San Diego, Calif.)·2018
Same author

Development of an Escherichia coli-based biocatalytic system for the efficient synthesis of N-acetyl-D-neuraminic acid.

Metabolic engineering·2018
Same author

High-Quality AZO/Au/AZO Sandwich Film with Ultralow Optical Loss and Resistivity for Transparent Flexible Electrodes.

ACS applied materials & interfaces·2018
Same journal

[Experimental teaching design of instrument analysis for undergraduate study： detection of bifenthrin pesticide residues in tea by response surface methodology-optimized QuEChERS combined with gas chromatography-mass spectrometry].

Se pu = Chinese journal of chromatography·2026
Same journal

[Case-based teaching with both positive and negative examples in undergraduate analytical chemistry laboratory： determination of six nitrogen-containing pesticides in environmental samples by solid-phase microextraction-high performance liquid chromatography].

Se pu = Chinese journal of chromatography·2026
Same journal

[Revolution teaching practice in sample pretreatment supported by large-scale instrument： a case study of magnetic covalent organic framework enriching organic ultraviolet filters in seawater].

Se pu = Chinese journal of chromatography·2026
Same journal

[Determination of 40 carbonyl compounds in ambient air by high performance liquid chromatography-triple quadrupole mass spectrometry].

Se pu = Chinese journal of chromatography·2026
Same journal

[Determination of 22 organophosphates and their metabolites and 11 phthalate metabolites in human whole blood by ultra performance liquid chromatography-tandem mass spectrometry].

Se pu = Chinese journal of chromatography·2026
Same journal

[Determination of <i>S-</i>enantiomer in linagliptin by supercritical fluid chromatography-ultraviolet detection].

Se pu = Chinese journal of chromatography·2026
See all related articles

Related Experiment Video

Updated: May 30, 2026

Microscale Vortex-assisted Electroporator for Sequential Molecular Delivery
10:51

Microscale Vortex-assisted Electroporator for Sequential Molecular Delivery

Published on: August 7, 2014

[Progress in micro free flow electrophoresis].

Pingli Wang1, Zhen Liang, Lihua Zhang

  • 1National Chromatographic R. & A. Center, Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China.

Se Pu = Chinese Journal of Chromatography
|July 21, 2011
PubMed
Summary
This summary is machine-generated.

Micro free flow electrophoresis (micro-FFE) offers continuous separation for biomolecules like cells and proteins. This review highlights recent advancements in micro-chip design, separation techniques, and applications, while also exploring future potential.

More Related Videos

Electrophoretic Separation of Proteins
08:17

Electrophoretic Separation of Proteins

Published on: June 12, 2008

Label-free Isolation and Enrichment of Cells Through Contactless Dielectrophoresis
10:38

Label-free Isolation and Enrichment of Cells Through Contactless Dielectrophoresis

Published on: September 3, 2013

Related Experiment Videos

Last Updated: May 30, 2026

Microscale Vortex-assisted Electroporator for Sequential Molecular Delivery
10:51

Microscale Vortex-assisted Electroporator for Sequential Molecular Delivery

Published on: August 7, 2014

Electrophoretic Separation of Proteins
08:17

Electrophoretic Separation of Proteins

Published on: June 12, 2008

Label-free Isolation and Enrichment of Cells Through Contactless Dielectrophoresis
10:38

Label-free Isolation and Enrichment of Cells Through Contactless Dielectrophoresis

Published on: September 3, 2013

Area of Science:

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry

Context:

  • Micro free flow electrophoresis (micro-FFE) is a continuous micro-separation technique.
  • It is utilized for analyzing biomolecules such as cells, subcellular components, and proteins.

Purpose:

  • This review summarizes recent progress in micro-FFE.
  • Emphasis is placed on microchip design, separation modes, and applications.
  • Future developments in micro-FFE are also prospected.

Summary:

  • Recent advancements in micro-FFE technology are reviewed.
  • Key areas covered include microchip design innovations, diverse separation modes, and established applications in biomolecule analysis.
  • The review also offers insights into the future trajectory of micro-FFE development.

Impact:

  • Provides a comprehensive overview of the current state of micro-FFE.
  • Highlights the technique's utility in biomolecule analysis.
  • Informs future research and development in microfluidic separation technologies.