Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: May 30, 2026

Real-time Tracking of DNA Fragment Separation by Smartphone
06:58

Real-time Tracking of DNA Fragment Separation by Smartphone

Published on: June 1, 2017

DNA separation in nanowall array chips.

Takao Yasui1, Noritada Kaji, Ryo Ogawa

  • 1Department of Applied Chemistry, Graduate School of Engineering, Nagoya University, Nagoya 464-8603, Japan. yasui.takao@e.mbox.nagoya-u.ac.jp

Analytical Chemistry
|July 21, 2011
PubMed
Summary

Researchers developed a nanowall array chip for rapid DNA separation. This novel method separates DNA fragments based on their dynamic behavior, offering a new approach beyond traditional gel electrophoresis.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Detection of H5 subtype avian influenza virus in avian oropharyngeal swab samples using a microfluidic non-competitive fluorescence polarization immunoassay.

The Analyst·2026
Same author

Dual-readout villus-like scaffold microfluidic platform for quantitative analysis of gut microbiota formation under perfusion.

The Analyst·2026
Same author

Targeted Intracellular Delivery of Amino Acids to Trophoblast Cells Reveals Proteomic Signatures of Cellular Utilisation.

Biomolecules·2026
Same author

Determination of okadaic acid in scallop using microfluidic-based fluorescence polarization immunoassay.

Analytical sciences : the international journal of the Japan Society for Analytical Chemistry·2026
Same author

Structural Elucidation of Azo and Quinoneimine Products Formed in Diazonium-Based Color Reactions of Cannabinoids.

Molecules (Basel, Switzerland)·2026
Same author

Consecutive Mechano-Immunophenotyping of Single Cells in a Photopatterned Microfluidic Channel.

ACS omega·2026

Area of Science:

  • Nanotechnology
  • Molecular Biology
  • Biophysics

Background:

  • Traditional DNA separation methods like gel electrophoresis are time-consuming.
  • Existing nanostructure-based separation techniques (nanopillars, nanoparticles) have limitations.
  • Understanding DNA dynamics in confined environments is crucial for developing advanced separation technologies.

Purpose of the Study:

  • To fabricate a nanowall array chip for efficient DNA fragment separation.
  • To investigate the mechanism of DNA separation in the nanowall array structure.
  • To compare the separation performance with existing DNA separation techniques.

Main Methods:

  • Fabrication of a nanowall array structure on a quartz chip.
  • Applying electric voltage for DNA fragment separation.

More Related Videos

Stable DNA Motifs, 1D and 2D Nanostructures Constructed from Small Circular DNA Molecules
09:32

Stable DNA Motifs, 1D and 2D Nanostructures Constructed from Small Circular DNA Molecules

Published on: April 12, 2019

Gel-seq: A Method for Simultaneous Sequencing Library Preparation of DNA and RNA Using Hydrogel Matrices
09:19

Gel-seq: A Method for Simultaneous Sequencing Library Preparation of DNA and RNA Using Hydrogel Matrices

Published on: March 26, 2018

Related Experiment Videos

Last Updated: May 30, 2026

Real-time Tracking of DNA Fragment Separation by Smartphone
06:58

Real-time Tracking of DNA Fragment Separation by Smartphone

Published on: June 1, 2017

Stable DNA Motifs, 1D and 2D Nanostructures Constructed from Small Circular DNA Molecules
09:32

Stable DNA Motifs, 1D and 2D Nanostructures Constructed from Small Circular DNA Molecules

Published on: April 12, 2019

Gel-seq: A Method for Simultaneous Sequencing Library Preparation of DNA and RNA Using Hydrogel Matrices
09:19

Gel-seq: A Method for Simultaneous Sequencing Library Preparation of DNA and RNA Using Hydrogel Matrices

Published on: March 26, 2018

  • Direct observation of single-DNA molecular dynamics within the nanowall array.
  • Numerical fitting of observed DNA molecular dynamics.

    • Main Results:

    • Achieved separation of a DNA mixture (48.5 bp and 1 kbp fragments) in 30 seconds.
    • Observed that longer DNA fragments migrate faster than shorter ones, differing from gel electrophoresis.
    • Identified confined elongation and relaxation-recoiling dynamics of single DNA molecules.
    • Demonstrated that DNA migration speed is influenced by elongation and recoiling behavior.

    Conclusions:

    • The nanowall array chip provides a novel and rapid method for DNA separation.
    • DNA separation is governed by the interplay between transverse movement and relaxation-recoiling dynamics.
    • This mechanism differs from entropic trapping and offers a new paradigm for DNA analysis.