Related Experiment Video
Updated: May 30, 2026

Flow Cytometric Analysis of Biomarkers for Detecting Human Sperm Functional Defects
Published on: April 21, 2022
What is normal semen quality? On the use and abuse of reference limits for the interpretation of semen analysis
1Department of Medicine, Centre for Andrology and Sexual Medicine, Karolinska University Hospital and Karolinska Institutet, Huddinge, Stockholm, Sweden. Lars.Bjorndahl@ki.se
Abstract:
Semen analysis is the corner stone in the basic evaluation of the man in the subfertile couple. The recent WHO manual identifies and recommends robust, controllable methods. However, the distribution of results from recent fathers and men in the general population introduces significant problems. This review discusses the pitfalls with reference limits and the proper use of such limits for the interpretation of semen analysis results. It is critical to understand the statistical basis upon which the assumptions of reference ranges and cut-off limits are built and the importance of standardising methods and practical laboratory training. These are indispensable for qualitative laboratory work as well as for future prospective studies aimed at providing prognostic information for spontaneous pregnancies and successful Assisted Reproductive Techniques (ART) treatment. Proper understanding of biological and physiological variability is also essential for the correct interpretation of semen analysis results. Understanding all the factors influencing semen analyses is of great importance for the development of the entire field of reproductive medicine.
Related Concept Videos
Sperm Structure and Semen Composition
Serum Studies: Renal Function Tests
Urine Studies I: Urinalysis
Unusual Results
According to the range rule of thumb, any value above or below two standard deviations, 2σ from the mean, μ is considered unusual.
Maximum unusual value = μ + 2σ
Minimum unusual value...
Infertility in Males
Quality Assurance

