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Updated: May 30, 2026

Intravital Imaging of the Mouse Popliteal Lymph Node
Published on: February 8, 2012
Comparison of computed tomographic and radiographic popliteal lymphangiography in normal dogs
Ameet Singh1, Brigitte A Brisson, Stephanie Nykamp
1Department of Clinical Studies, Ontario Veterinary College, University of Guelph, Guelph, ON, Canada.
Objective:
To (1) describe computed tomographic (CT) popliteal lymphangiography; (2) compare the number of thoracic duct (TD) branches detected by CT and by radiography after popliteal lymphangiography; and (3) to compare the number of branches detected after left and right popliteal lymphangiography.
Study Design:
Experimental study.
Animals:
Adult dogs (n=6).
Methods:
A randomly selected popliteal lymph node was percutaneously injected with 12 L iodinated contrast medium through a 25-g butterfly catheter over 4-5 minutes. Lateral and ventrodorsal (VD) thoracic radiograph projections and thoracic CT were performed. The procedure was repeated using the contralateral lymph node after a 48-72 hours washout period.
Results:
One dog had TD branches visible on CT but not on radiographs. A significantly greater number of TD branches were observed with CT popliteal lymphangiography compared with lateral and VD radiographic popliteal lymphangiography (P=.003 and P<.001, respectively). The number of visible TD branches observed between the 6th thoracic and 1st lumbar vertebrae were not significantly different in these dogs (P=.146). A significant difference in number of TD branches observed was not found after left or right popliteal lymph node injection (P=.097).
Conclusions:
CT popliteal lymphangiography consistently identified a greater number of TD branches when compared with radiographic popliteal lymphangiography. Injection of either popliteal lymph node resulted in the same number of TD branches being observed.
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