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Related Concept Videos

Transcription in Prokaryotes01:28

Transcription in Prokaryotes

Transcription is a highly regulated process that converts genetic information into RNA molecules. The transcription cycle is divided into three key stages: initiation, elongation, and termination, each driven by specific molecular mechanisms.Initiation of TranscriptionIn bacteria, transcription begins when the RNA polymerase core enzyme associates with a sigma factor to form a holoenzyme. For example, the E. coli sigma factor called σ70 forms a holoenzyme, which recognizes the -10 (Pribnow box)...
Transcription Attenuation in Prokaryotes02:42

Transcription Attenuation in Prokaryotes

Transcriptional attenuation occurs when RNA transcription is prematurely terminated due to the formation of a terminator mRNA hairpin structure.  Bacteria use these hairpins to regulate the transcription process and control the synthesis of several amino acids including histidine, lysine, threonine, and phenylalanine. Transcription attenuation takes place in the non-coding regions of mRNA.
There are several different mechanisms used to attenuate transcription. In ribosome mediated...
Bacterial Transcription01:53

Bacterial Transcription

RNA polymerase (RNAP) carries out DNA-dependent RNA synthesis in both bacteria and eukaryotes. Bacteria do not have a membrane-bound nucleus. So, transcription and translation occur simultaneously, on the same DNA template.
Transcription can be divided into three main stages, each involving distinct DNA sequences to guide the polymerase. These are:
Yeast Signaling01:28

Yeast Signaling

Yeasts are single-celled organisms, but unlike bacteria, they are eukaryotes (cells with a nucleus). Cell signaling in yeast is similar to signaling in other eukaryotic cells. A ligand, such as a protein or a small molecule released from a yeast cell, attaches to a receptor on the cell surface. The binding stimulates second-messenger kinases to activate or inactivate transcription factors that further regulate gene expression. Many of the yeast intracellular signaling cascades have similar...
Prokaryotic Transcriptional Activators and Repressors01:58

Prokaryotic Transcriptional Activators and Repressors

The organization of prokaryotic genes in their genome is notably different from that of eukaryotes. Prokaryotic genes are organized, such that the genes for proteins involved in the same biochemical process or function are located together in groups. This group of genes, along with their regulatory elements, are collectively known as an operon. The functional genes in an operon are transcribed together to give a single strand of mRNA known as polycistronic mRNA.
Transcription of prokaryotic...
Prokaryotic Transcriptional Activators and Repressors01:58

Prokaryotic Transcriptional Activators and Repressors

The organization of prokaryotic genes in their genome is notably different from that of eukaryotes. Prokaryotic genes are organized, such that the genes for proteins involved in the same biochemical process or function are located together in groups. This group of genes, along with their regulatory elements, are collectively known as an operon. The functional genes in an operon are transcribed together to give a single strand of mRNA known as polycistronic mRNA.
Transcription of prokaryotic...

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Related Experiment Video

Updated: May 30, 2026

Analysis of Termination of Transcription Using BrUTP-strand-specific Transcription Run-on (TRO) Approach
12:12

Analysis of Termination of Transcription Using BrUTP-strand-specific Transcription Run-on (TRO) Approach

Published on: March 12, 2017

Pervasive transcription - Lessons from yeast.

Mathieu Tisseur1, Marta Kwapisz, Antonin Morillon

  • 1ncRNA, Epigenetic and Genome Fluidity, Institut Curie, Centre de Recherche, CNRS UMR3244, Université Pierre et Marie Curie, 26 rue d'Ulm, 75248 Paris Cedex 05, France.

Biochimie
|July 21, 2011
PubMed
Summary

Pervasive transcription generates non-coding RNAs (ncRNAs) in eukaryotic genomes. Saccharomyces cerevisiae is a key model for studying large ncRNAs and their regulation by RNA decay and nucleosome positioning.

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In Vivo Monitoring of Transcriptional Activity During Metabolic Transition Using a Bioluminescent Reporter in Yeast

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Area of Science:

  • Genomics
  • Molecular Biology
  • RNA Biology

Background:

  • Eukaryotic genomes exhibit pervasive transcription, producing various non-coding RNAs (ncRNAs).
  • Regulatory ncRNAs play crucial roles in gene expression and chromatin organization.
  • The yeast Saccharomyces cerevisiae is increasingly recognized for its contributions to understanding these processes.

Purpose of the Study:

  • To review recent findings on pervasive transcription in Saccharomyces cerevisiae.
  • To highlight the regulatory mechanisms controlling pervasive transcription, including RNA decay and nucleosome positioning.
  • To propose Saccharomyces cerevisiae as a valuable model for studying large ncRNAs.

Main Methods:

  • Literature review of recent reports on pervasive transcription in Saccharomyces cerevisiae.
  • Analysis of studies focusing on RNA decay pathways and nucleosome positioning in yeast.
  • Examination of examples of antisense-mediated transcriptional silencing.

Main Results:

  • Saccharomyces cerevisiae's genome supports pervasive transcription.
  • RNA decay pathways and nucleosome positioning are critical regulators of this transcription.
  • The yeast model has yielded significant insights into antisense-mediated transcriptional silencing.

Conclusions:

  • Saccharomyces cerevisiae is a powerful model organism for investigating large ncRNAs.
  • Understanding pervasive transcription in yeast provides fundamental insights into eukaryotic gene regulation.
  • Further research in yeast will continue to elucidate the roles of ncRNAs in gene control.