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Updated: May 30, 2026
Optimizing Tubulin Yield from Porcine Brain Tissue
Published on: October 11, 2024
Purification of tubulin from porcine brain
Christopher Gell1, Claire T Friel, Barbara Borgonovo
1Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany. gell@mpi-cbg.de
Abstract:
Microtubules, polymers of the heterodimeric protein αβ-tubulin, give shape to cells and are the tracks for vesicle transport and chromosome segregation. In vitro assays to study microtubule functions and their regulation by microtubule-associated proteins require the availability of purified αβ-tubulin. In this chapter, we describe the process of purification of heterodimeric αβ-tubulin from porcine brain.
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