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Updated: May 30, 2026
A Customizable Approach for the Enzymatic Production and Purification of Diterpenoid Natural Products
Published on: October 4, 2019
Preparative isolation, fast centrifugal partition chromatography purification and biological activity of
Sylvie Morel1, Anne Landreau, Van Hung Nguyen
1SONAS EA 921, IFR 149, Quasav UFR des Sciences Pharmaceutiques et d'Ingénierie de la Santé, 16 Bd Daviers, 49100 Angers, France.
Introduction:
The Derris genus is known to contain flavonoid derivatives, including prenylated flavanones and isoflavonoids such as rotenoids, which are generally associated with significant biological activity.
Objective:
To develop an efficient preparative isolation procedure for bioactive cajaflavanone.
Methodology:
Fast centrifugal partition chromatography (FCPC) was optimised to purify cajaflavanone from Derris ferruginea stems in a single step as compared to fractionation from the cyclohexane extract by successive conventional solid-liquid chromatography procedures. The purification yield, purity, time and solvent consumption per procedure are described. The anti-fungal, anti-bacterial, anti-leishmanial, anti-plasmodial, anti-oxidant activities and the inhibition of advanced glycation end-products (AGEs) by cajaflavanone accumulation are described.
Results:
FCPC enabled cajaflavanone purification in a single separation step, yielding sufficient quantities to perform in vitro biological screening. Interestingly, cajaflavanone had an inhibitory effect on the formation of AGEs, without displaying any in vitro anti-oxidant activity.
Conclusion:
A simple and efficient procedure, in comparison with other preparative methods, for bioactive cajaflavone purification has been developed using FCPC.
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