Related Experiment Video
Updated: May 30, 2026
09:46
Megakaryocyte Differentiation and Platelet Formation from Human Cord Blood-derived CD34+ Cells
Published on: December 27, 2017
[Polyclonal antibodies against human cell-surface antigen CD34]
Tsitologiia I Genetika
|July 22, 2011
Summary
Researchers developed an efficient method to create polyclonal antibodies against the human CD34 antigen. These antibodies, generated using a recombinant protein, are effective for CD34+ cell phenotyping in various assays.
Area of Science:
- Immunology
- Biotechnology
- Cell Biology
Background:
- The human CD34 antigen is a crucial marker for hematopoietic stem cells.
- Developing specific and reliable antibodies for CD34 detection is essential for cell analysis.
Purpose of the Study:
- To establish an efficient and cost-effective laboratory method for generating polyclonal antibodies against human CD34.
- To validate the utility of these antibodies in immunocytochemistry and flow cytometry.
Main Methods:
- Production of a cloned, refolded, and purified recombinant extracellular fragment of the human CD34 antigen from Escherichia coli.
- Immunization of mice with the unglycosylated, truncated recombinant CD34 protein.
- Application of generated polyclonal antibodies in immunocytochemistry and flow cytometry assays.
Main Results:
- The recombinant CD34 fragment retained immunogenic determinants of the native cell-surface antigen.
- Polyclonal antibodies specific for native human CD34 were successfully elicited.
- The generated antibodies demonstrated applicability for phenotyping CD34+ cells.
Conclusions:
- An efficient and inexpensive method for producing CD34-specific polyclonal antibodies was established.
- Recombinant CD34 protein is a viable immunogen for antibody generation.
- These antibodies are suitable tools for CD34+ cell identification and analysis.
