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Updated: May 30, 2026

Rescue of Recombinant Newcastle Disease Virus from cDNA
Published on: October 11, 2013
Artificial recombination may influence the evolutionary analysis of Newcastle disease virus
Qingqing Song1, Yongzhong Cao, Qun Li
1College of Veterinary Medicine, Yangzhou University, Yangzhou, China.
Abstract:
The recombination rate in Newcastle disease virus (NDV) was as high as 10% in RDP analysis with full-length NDV genome sequences available in GenBank. We found that two NDV strains, China/Guangxi09/2003 and NDV/03/018, previously reported as recombinants, failed to show any evidence of recombination upon complete genome resequencing. Furthermore, we were able to reproduce artificial recombination by amplification of the M gene in a mixed sample of strains LaSota and ZJ1. It appears that the recombination of NDV is not as common as has been reported. NDV sequences in GenBank should be analyzed with caution during bioinformatic analyses for natural recombination events.
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