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Updated: May 30, 2026

DNA-Tethered RNA Polymerase for Programmable In vitro Transcription and Molecular Computation
Published on: December 29, 2021
A novel eukaryote-made thermostable DNA polymerase which is free from bacterial DNA contamination
Hideki Niimi1, Masashi Mori, Homare Tabata
1Clinical Laboratory Center, Toyama University Hospital, 2630 Sugitani Toyama 930-0194, Japan. hiniimi@med.u-toyama.ac.jp
Abstract:
To achieve the production of a thermostable DNA polymerase free from bacterial DNA contamination, we developed eukaryote-made thermostable DNA (Taq) polymerase. The novel eukaryote-made thermostable DNA polymerase resolves the problem of contaminating bacterial DNA in conventional bacterially made thermostable DNA polymerase as a result of its manufacture and incomplete purification. Using eukaryote-made thermostable DNA polymerase, the sensitive and reliable detection of bacteria becomes feasible for large fields, thereby making the development of a wide range of powerful applications possible.
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