Related Experiment Video
Updated: May 30, 2026

Enrichment and Detection of Clostridium perfringens Toxinotypes in Retail Food Samples
Published on: October 18, 2019
Comparison of a commercially available repetitive-element PCR system (DiversiLab) with PCR ribotyping for typing of
C Eckert1, J Van Broeck, P Spigaglia
1ER8, Université Pierre et Marie Curie, Paris, France. catherine.eckert@sat.aphp.fr
Abstract:
This study compared a repetitive-element PCR (rep-PCR) method (DiversiLab system) to PCR ribotyping. The discriminatory power of rep-PCR was 0.997. Among the PCR ribotype 027 isolates tested, different rep types could be distinguished. rep-PCR showed a higher discriminatory power than PCR ribotyping. Nevertheless, this method requires technical skill, and visual interpretation of rep-PCR fingerprint patterns may be difficult.
Related Concept Videos
Modern Molecular Taxonomy
Automated Microbial Diagnostics

