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Related Experiment Video

Updated: May 30, 2026

Visualizing Dengue Virus through Alexa Fluor Labeling
09:11

Visualizing Dengue Virus through Alexa Fluor Labeling

Published on: July 9, 2011

Visualizing dengue virus through Alexa Fluor labeling.

Summer Zhang1, Hwee Cheng Tan, Eng Eong Ooi

  • 1Defence Medical and Environmental Research Institute, DSO National Laboratories.

Journal of Visualized Experiments : Jove
|July 22, 2011
PubMed
Summary
This summary is machine-generated.

Researchers developed a simple method to label dengue virus using Alexa Fluor dye without dimethyl sulfoxide, ensuring virus viability. This technique aids in studying dengue virus-host interactions and pathogenesis.

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Last Updated: May 30, 2026

Visualizing Dengue Virus through Alexa Fluor Labeling
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06:28

Flow Virometry to Analyze Antigenic Spectra of Virions and Extracellular Vesicles

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Area of Science:

  • Virology
  • Molecular Biology
  • Biotechnology

Background:

  • Early virus-cell interactions significantly impact infection outcomes and disease pathogenesis.
  • Understanding these interactions is crucial for developing effective vaccines and therapeutics.
  • Current methods for studying virus-host interactions are limited.

Purpose of the Study:

  • To develop a simple, effective method for labeling dengue virus for visualization and study.
  • To improve the understanding of dengue virus pathogenesis through advanced imaging techniques.
  • To create a tool that can help reduce the annual incidence of dengue infections.

Main Methods:

  • Developed a novel method for labeling dengue virus using Alexa Fluor succinimidyl ester dye dissolved in sodium bicarbonate buffer.
  • Excluded the use of dimethyl sulfoxide, which can inhibit viral infection and cause cell toxicity.
  • Utilized immunofluorescent microscopy to visualize the labeled virus.

Main Results:

  • The developed labeling method yielded highly viable dengue virus.
  • The Alexa Fluor dye conjugation did not impede virus recognition by antibodies or host cell receptors.
  • The method demonstrated the potential for superior photostability and pH insensitivity compared to traditional dyes.

Conclusions:

  • This simple, DMSO-free labeling technique enhances dengue virus viability and maintains its infectivity.
  • The method is suitable for studying virus cellular uptake and endosomal transport.
  • This approach offers valuable applications for virological research and dengue pathogenesis studies.