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Related Experiment Video

Updated: May 30, 2026

Mouse Bladder Wall Injection
03:48

Mouse Bladder Wall Injection

Published on: July 12, 2011

Mouse bladder wall injection.

Chi-Ling Fu1, Charity A Apelo, Baldemar Torres

  • 1Department of Urology, Stanford University School of Medicin, USA.

Journal of Visualized Experiments : Jove
|July 22, 2011
PubMed
Summary
This summary is machine-generated.

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Mouse bladder wall injection is a valuable technique for studying bladder stem cell, smooth muscle, and cancer biology. This microsurgical method involves precise intramural injection into the bladder dome for orthotopic research.

Area of Science:

  • Urology
  • Surgical Techniques
  • Biomedical Research

Background:

  • Orthotopic mouse bladder models are crucial for studying bladder physiology and pathology.
  • Existing techniques may have limitations in precisely targeting bladder wall phenomena.

Purpose of the Study:

  • To describe a standardized and effective technique for mouse bladder wall injection.
  • To facilitate research in bladder stem cell biology, smooth muscle function, and cancer biology using orthotopic models.

Main Methods:

  • The procedure involves aseptic surgical preparation of the abdominal area and sterilization of instruments.
  • Mice are anesthetized with isoflurane, and the bladder is exposed via a midline abdominal incision.
  • Cell suspensions are intramurally injected into the bladder dome using a fine-gauge needle and syringe, followed by wound closure and recovery.

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Related Experiment Videos

Last Updated: May 30, 2026

Mouse Bladder Wall Injection
03:48

Mouse Bladder Wall Injection

Published on: July 12, 2011

Minimally Invasive Establishment of Murine Orthotopic Bladder Xenografts
08:15

Minimally Invasive Establishment of Murine Orthotopic Bladder Xenografts

Published on: February 11, 2014

Transurethral Instillation Procedure in Adult Male Mouse
04:01

Transurethral Instillation Procedure in Adult Male Mouse

Published on: November 2, 2017

Main Results:

  • The described technique allows for precise, orthotopic delivery of cell suspensions into the mouse bladder wall.
  • Successful implementation enables the study of various bladder-related biological processes in vivo.
  • The technique is characterized as a delicate microsurgical procedure requiring practice for mastery.

Conclusions:

  • Mouse bladder wall injection is a feasible and valuable microsurgical technique for orthotopic bladder research.
  • This method supports advancements in understanding bladder stem cells, smooth muscle, and cancer biology.
  • Mastery of this technique enhances the reliability of orthotopic bladder studies.