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DNA Isolation

DNA Isolation

DNA isolation protocols can be fast and straightforward or complex and time-consuming depending on the type and quality of DNA required for further processing. For example, plasmid DNA extraction is a bit more complicated than genomic DNA extraction because of the need for an appropriate lysis method to separate plasmid DNA from gDNA during isolation. However, for specific applications, such as long-range DNA sequencing that require a good yield of high- quality DNA samples, we need to follow...

DNA Isolation

DNA Isolation

DNA from cells is required for many biotechnology and research applications, such as molecular cloning. To remove and purify DNA from cells, researchers use various methods of DNA extraction. While the specifics of different protocols may vary, some general concepts underlie the process of DNA extraction.

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Related Experiment Video

Updated: May 30, 2026

Biolistic Transformation of a Fluorescent Tagged Gene into the Opportunistic Fungal Pathogen Cryptococcus neoformans

Biolistic Transformation of a Fluorescent Tagged Gene into the Opportunistic Fungal Pathogen Cryptococcus neoformans

Published on: March 19, 2015

A rapid and easy method for the DNA extraction from Cryptococcus neoformans.

Fatma Mseddi¹, Mohammed Ali Jarboui, Amira Sellami

¹Laboratoire de Biologie Moléculaire Parasitaire et Fongique, Faculté de Médecine de, 3029, Sfax, Tunisia. ali.ayadi@rns.tn.

Biological Procedures Online

|July 23, 2011

Summary

This summary is machine-generated.

We developed a fast DNA isolation method for Cryptococcus, a fungus with a tough capsule. This new urea and Chelex resin technique simplifies DNA extraction for researchers.

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Last Updated: May 30, 2026

Biolistic Transformation of a Fluorescent Tagged Gene into the Opportunistic Fungal Pathogen Cryptococcus neoformans

Biolistic Transformation of a Fluorescent Tagged Gene into the Opportunistic Fungal Pathogen Cryptococcus neoformans

Published on: March 19, 2015

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Area of Science:

Microbiology
Molecular Biology
Biotechnology

Background:

Cryptococcus neoformans possesses a thick, resistant capsule, complicating standard DNA isolation procedures.
Efficient DNA extraction is crucial for molecular studies, diagnostics, and strain typing of C. neoformans.

Purpose of the Study:

To optimize a novel and rapid DNA isolation method for Cryptococcus species.
To overcome the challenges posed by the organism's capsule during DNA extraction.

Main Methods:

A short urea treatment was employed to weaken the fungal capsule.
DNA was subsequently isolated using a rapid Chelex resin suspension method.

Main Results:

The optimized procedure provides a simplified and faster alternative to existing DNA isolation methods for C. neoformans.
Successful DNA extraction was achieved, facilitating downstream molecular applications.

Conclusions:

This new method offers an efficient approach for DNA isolation from Cryptococcus.
The technique's simplicity and speed make it valuable for clinical and research laboratories.