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Related Concept Videos

Aryldiazonium Salts to Azo Dyes: Diazo Coupling01:11

Aryldiazonium Salts to Azo Dyes: Diazo Coupling

The reaction of weakly electrophilic aryldiazonium (also called arenediazonium) salts with highly activated aromatic compounds leads to the formation of products with an —N=N— link, called an azo linkage. This reaction, presented in Figure 1, is known as diazo coupling and occurs without the loss of the nitrogen atoms of the aryldiazonium salt. Highly activated aromatic compounds such as phenols or arylamines favor the diazo coupling reaction. The coupling generally occurs at the para position.
Labeling DNA Probes03:31

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...

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Updated: May 30, 2026

Identification of Small Molecule-binding Proteins in a Native Cellular Environment by Live-cell Photoaffinity Labeling
10:49

Identification of Small Molecule-binding Proteins in a Native Cellular Environment by Live-cell Photoaffinity Labeling

Published on: September 20, 2016

Diazirine based photoaffinity labeling.

Luba Dubinsky1, Bastiaan P Krom, Michael M Meijler

  • 1Department of Chemistry and National Institute for Biotechnology in the Negev, Ben-Gurion University of the Negev, Be'er Sheva, Israel.

Bioorganic & Medicinal Chemistry
|July 23, 2011
PubMed
Summary
This summary is machine-generated.

Diazirines are small photoreactive molecules used in photoaffinity labeling. Recent advances highlight their utility in studying molecular interactions and identifying unknown proteins.

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Area of Science:

  • Chemical Biology
  • Biochemistry
  • Molecular Biology

Background:

  • Diazirines are small, nitrogen-containing cyclic compounds.
  • Upon light irradiation, diazirines generate highly reactive carbenes.
  • Carbenes are versatile intermediates in organic chemistry.

Purpose of the Study:

  • To review recent advancements in the application of diazirines.
  • To highlight the use of diazirines in photoaffinity labeling techniques.
  • To discuss the role of diazirines in biological research.

Main Methods:

  • Photoaffinity labeling using diazirine-based probes.
  • Spectroscopic analysis of carbene intermediates.
  • Biochemical assays to study molecular interactions.
  • Protein identification and isolation techniques.

Main Results:

  • Diazirines enable efficient photoactivation and carbene generation.
  • Photoaffinity labeling with diazirines has advanced the study of ligand-receptor interactions.
  • Diazirines aid in identifying binding partners and characterizing protein complexes.
  • Recent studies demonstrate novel applications in chemical biology.

Conclusions:

  • Diazirines are powerful tools for photoaffinity labeling.
  • Their small size and reactivity offer unique advantages in biological studies.
  • Continued development promises further expansion of their applications in molecular and chemical biology.